FROM IMAGES TO ANSWERS April 2005

Agenda 15 minutesImage Acquisition New Tools Single, Multiple, Time Lapse, Z-Stacks 15 minutesCalibration & Annotation 15 minutesImage Processing Illumination, Filters, Alignment, EDF 15 minutesImage Analysis Manual & Automated Measurements Questions & Answers

Image Pro Plus 5.1 FROM IMAGES TO ANSWERS

Image-Pro Family Software Series Perfect for Basic Imaging or Capture Stations More Advanced than Image- Pro Express, Includes Support for Plug-Ins The Ultimate Imaging Package, Includes Support for Macros and Plug-Ins

Image-Pro Family Bundles Image-Pro Advanced Microscopy Suite This complete solution for high-level microscopy analysis and acquisition combines Image-Pro Plus 5.1 with powerful tools for multi-dimensional microscope control, image deconvolution, and 3D rendering. Image-Pro Plus 5.1 Image-Pro Multi-Dimensional Acquisition Ideal for automated microscopy work, this package combines Image-Pro Plus 5.1 with tools for multi-dimensional microscope control and acquisition.Image-Pro Plus 5.1 Image-Pro 3D Suite Ideal for deconvolution and interactive control and analysis of volume stacks, this package combines Image-Pro Plus 5.1 with deconvolution and rendering tools.Image-Pro Plus 5.1 Image-Pro Microscope Control The Image-Pro Microscope Control (MC) package combines Image-Pro Plus Version 5.1 with tools to control and program all major automated microscopes.Image-Pro Plus Version 5.1 Image-Pro 3D Discovery Perfect for confocal microscopy, the Image-Pro 3D Discovery package combines Image-Pro Discovery 5.1 with tools to provide full interaction and analysis of all 3D volumes. Image-Pro Analyzer 5 or 10 Pack Ideal for networked users who want advanced analysis tools but do not need image capture functionality at each work station.

Image Pro Plus 5.1 Features Count Size Extended Depth of Field

Image Pro Camera Control Advanced Camera Control Dynamic Auto Contrast Live Histogram Transparency Mode Time Lapse Configurable

Image Pro Microscope Control Nikon TE 2000 E2, Nikon 90i, Nikon Focus and Nikon E1000M Software control of stages, filter wheels, shutters, z-motors, microscopes Ludl, Marzhauzer, Prior, ASI, Objective Imaging Sutter and Physik

Image Pro Multi-Dimensional Acquisition

Image Pro 3D Suite 3D Suite Includes: No Neighbor, Nearest Neighbor Haze Removal and Inverse Filter Haze Restoration Algorithms An Option is Available which Includes 2D Blind and 3D Blind Deconvolution Algorithms These Algorithms are not Appropriate for Confocal Images

Image Pro 3D Suite 3D reconstruction, visualization and volumetric and spatial measurements Major Features –Free Rotation of Image Stacks –Extensive Animation & Display Tools –Orthogonal & Oblique Sectioning –Numerous Spatial & Volumetric Measurements –Time Resolved Tracking of Objects

Pro-Series MV-Pro & MV-Lite Capture Kits Board, Cable & Driver Included Works with Image Pro Express, Discovery or Plus Works with Most Video Cameras Specialized Cables also Available

Pro-Series Turboscan Fast mosaic acquisition and display Based on OASIS-4i and OASIS image controller hardware Solutions for digital and analog cameras Stand-alone application and Image- Pro® capture driver

Pro-Series Turbo-Scan Surveyor Mosaic window Region of interest transfer to IPP Joystick stage and focus control IPP Live Preview Live current position marker OASIS Turboscan Video/Digital capture driver

Example - Mouse, Transverse Section 10X Objective / 1X C-mountCalibration: 0.46 µm/pixel Scan fields: 17 x 20, 340 total Scan area: 10.6 x 9.2 mm = 98 sq. mm Mosaic size: 1.4 GBActual mosaic resolution: 23,596 x 20,720 pixels Scan time (snake scan with predictive focus and shading correction): 42 secs (8 fps avg)

IQbase (Now Included with Image Pro Plus) Powering Image Informatics Manages Images and Data Drives Discovery Facilitates Collaboration Easy to Use Works the Way You Do Grows with Your Organization (Scalable)

Online Application and Product Support

Solutions Zone The Solutions Zone is a searchable online database containing: Device Drivers Macros Plug-in modules Application programs

Solutions Zone Elispot Analysis Piezo-Scan (driver for piezo objective positioner) Nerve Quantification FRET Analysis Batch Processing Customized Applications Dozens of Free Solutions Including

Marketing Materials Press Releases Comparison Sheets Advertisements Feature Data Sheets Posters

Online Support and Solutions Zone

Online Comparison / Information

Licensing Pricing & Policies Image Pro PlusPrice First Copy$4, Copies$3,000 Upgrade from 4.X (First Copy) $1,300 Upgrade from 4.X (2-250 Copies) $1,000 Upgrade from 3.X or Lower$3,250

Licensing Pricing & Policies Networks (Floating Site Licenses) Same Pricing as Individual Licenses* One Network Dongle Three or More Copies Same Version * One-Time Exchange Fee for Conversion from Individual Licenses to Network License

Licensing Pricing & Policies Network Installation Install Server Software on “Server” Install Client Software on all other Computers in Network Use Image Pro Plus Concurrently up to Licensed Quantity

Licensing Pricing & Policies Technical Support Plans 90-day Free Technical Support Extended Technical Support Available at Discounted Prices $749 per license per year $1,499 per 10 copies per year

Silver Spring, MD (Headquarters) Offices in: Boston, MA, Pittsburgh, PA & San Diego, CA Leiden, The Netherlands Goettingen, Germany Wokingham, United Kingdom Singapore, Republic of Singapore Tokyo, Japan Shanghai, China