Genomics: READING genome sequences ASSEMBLY of the sequence ANNOTATION of the sequence carry out dideoxy sequencing connect seqs. to make whole chromosomes.

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Presentation transcript:

Genomics: READING genome sequences ASSEMBLY of the sequence ANNOTATION of the sequence carry out dideoxy sequencing connect seqs. to make whole chromosomes find the genes! “FIRST” generation sequencing

M.L. Metzker Nature Genetics Reviews 11, 31. First, 5 slides, in order of Figs. Ion torrent Nanopore

5 Selected Technologies. 2 nd, 3 rd, and 4 th generation sequencing. Using Metzker, M.L. 2010, and T.P. Niedringhaus 2011Anal. Chem. 83, Solexa - - ->Illumina

Allows both directions: “pair-end”

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NONE USED IN ROCHE/LIFE 454 PYROSEQUENCING

Ion Torrent (BIU)

Pacific Biosciences

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Nanopore DNA sequencing

??? work

Illumina Solexa 2015 HiSeq 3000 and HiSeq 4000 are Illumina’s next iteration sequencing system for the current HiSeq HiSeq 3000 has single flow cell, while HiSeq 4000 has two flow cells. Illumina brings its patterened flow cell design to the HiSeq 3000 and HiSeq 4000 and makes the to deliver more data at lower cost than the current HiSeq Illumina claims that the HiSeq 4000 can sequence up to 12 genomes, 100 whole transcriptome samples, or 180 exomes in 3.5 days or less.