New Products 소개 1.Pel-Freez HLA-B*27 High Resolution Kit (SSP) 2.Pel-Freez KIR Genotyping Kit (SSP) 3.Pel-Freez Cytokine Genotyping Kit (SSP) 4.Dynal.

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New Products 소개 1.Pel-Freez HLA-B*27 High Resolution Kit (SSP) 2.Pel-Freez KIR Genotyping Kit (SSP) 3.Pel-Freez Cytokine Genotyping Kit (SSP) 4.Dynal SeCore (Sequence based typing)

HLA-B*27 High Resolution HLA-B*27 과 강직성 척추염 1. 강직성 척추염 ; 만성 진행성 다발성 관절염의 일종 ; 드물게 척추외상, 상부위장관출혈, 대동맥폐쇄부전증, 호흡부전, 아밀로이드성 신증 등의 합병증 발생 위험 2. 따라서 조기 발견과 치료가 중요 3. HLA-B*2705, 2402, 개의 아형이 강직성 척추염과 연관되었다고 보고 (Ramos and Lopez. HLA-B27 and spondyloarthritis. Tissue Antigens 2002:60:191~205.) 4. 보험수가 - B27 DNA (High Resolution) : 60,760 원 - B27 DNA (Low Resolution) : 28,400 원 - B27 Serology : 45,620 원

HLA-B*27 High Resolution Pel-Freez HLA-B*27 Kit 1. Kit 2. 특징 - Hot Start PCR methods 채용으로 Fidelity 높임 - Ready to Use Format - Premixed master mix - Fast - Easy Interpretation (UniMatch Plus interpretation software) DescriptionProduct Code No. PCR Reactions/Test Size B*27 Unitray tests

NK Cell (Natural Killer Cell) 체내에서 MHC Class I 이 결핍된 암세포 죽이고 전이 억제 MHC class I 에 특이적으로 결합하는 억제 수용체에 의해 NK cell 의 능력 부여 KIR 는 이 억제 수용체의 하나 KIR Genotyping

KIR (Killer cell immunoglobulin-like receptor) 암세포를 죽이고, 전이를 억제하는데 관여 ( 혈액종양내과 ) 최근의 연구에서, - allogenic bone marrow transplantation (BMT) rejection 및 - Graft Versus-host desease (GVHD) 와 연관이 있다고 보고 KIR Genotyping

DescriptionProduct Code No. PCR Reactions/Test Size KIR Genotyping SSP Kit BY2012 tests KIR Genotyping Pel-Freez KIR Genotyping 1. Kit 2. 특징 - Hot Start PCR methods 채용으로 Fidelity 높임 - Ready to Use Format - Premixed master mix - Fast - Easy Interpretation (UniMatch Plus interpretation software)

KIR Genotyping Pel-Freez KIR Genotyping 3. KIR Allele Specificity

NK Panel Family 1416 Samples Amplified on MJ Tetrad IHW1175 IHW1181 Well # 2DL1* DL2* DL3* DL4* DL5A*001, 2DL5B* M DL5A*001 2DL5B* DS1* DS2* DS3* DS4*00101/00102/002 2DS4*003 (KIR1D) 2DS5* DL1* DL2* DL3*001/00201/00202/003/004 3DS1* DP1*001/002/00301/ DP1*001/ Negative KIR Genotyping

왜 Cytokine Genotyping 이 필요한가 ? Cytokine Gene polymorphisms - cytokine 의 plasma level 과 관련 ; kidney, heart 또는 lung allografts 에서 생존률과 관련 ; hematopoietic stem cell transplantation 에서 GVHD 발생과 관련 이식 후 유도 생산 되는 cytokine 의 양에 따라 거부반응의 빈도가 좌우됨 Cytokine 의 생성량은 유전형에 따라 다름 Cytokine Genotyping

Which Cytokine? - IL1 alpha - IL1 beta - IL1R - IL1RA - IL2 - IL4 - IL4R - IL6 - IL10 - IL12 - TGF b1 - TNF a - IFN g Cytokine Genotyping

Courtesy J. Mytilineos 1. 이식 후 TGF-beta1 의 생성량은 TGF-beta1 의 genotype 에 의해 결정 2.C (Codon10) G (Codon25) 가 보다 높은 TGF-beta1 생성 3.TGF-beta1 의 생성량이 높을수록 이식 후 예후 불량 ; TGF-β1 genotyping in kidney transplantation (Korean J Clin Pathol 2001;21:225-30) TGF-beta1

DescriptionProduct Code No. PCR Reactions/Test Size Cytokine Genotyping Kit tests Pel-Freez Cytokine Genotyping 1. Kit 2. 특징 - Hot Start PCR methods 채용으로 Fidelity 높임 - Ready to Use Format - Premixed master mix - Fast - Easy Interpretation (UniMatch Plus interpretation software) Cytokine Genotyping

Pel-Freez Cytokine Gene Allele Frequencies Cytokine Genotyping

Cytokine Gene Allele Frequencies

왜 Sequence Based Genogyping 이 필요한가 ? High Resolution Genotyping 가능 Ambiguity 해결의 필요성 비혈연 골수이식의 증가 새로운 대립유전자 발견 SeCore (Sequence Based Genotyping)

SeCore 의 장점 Equipment Compatible - Multi-dye capillary system Identical Cycling Profiles for all Loci - 1 standard amplification program - 1 sequencing program ; ; reducing errors and improving efficiency Amplification Internal Controls - Supply control DNA for trouble shooting SeCore (Sequence Based Genotyping)