Protective Immunity Project Chris Larsen, MD, DPhil, PI Christine Martens, Ph.D. Project Manager Vicki Hertzberg, Ph.D. Director of Data Management and.

Slides:

Advertisements

Similar presentations

Florins A 1, Debacq C 1, Gillet N 1, Jean G 1, Thewis A 1, Schwartz-Cornil I 2, Bonneau M 2, Hay J 3, Asquith B 4, Burny A 1, Kettmann R 1 and Willems.

Advertisements

+ - Cell Sorting LASER. Cell Sorting + - LASER Zhang at al, Blood 2003, 102: Flow cytometry analysis of mouse fetal liver cells. Mouse fetal.

Supplementary Materials. Supplementary Figure S1. The effect of the freezing/thawing procedure on CD15 and CD33 marker staining on human PBMCs. Prostate.

Laser Flow Cytometry Forward Scatter indicates size Forward Scatter.

Date of download: 5/31/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Toll-like Receptors in Regulatory T Cells of Patients.

Multiplex analysis of cytokines Russell Garland KWS BioTest

Quantifying Mesenchymal Stem Cells in the Mononuclear Cell Fraction of Bone Marrow Samples Obtained for Cell Therapy M. Alvarez-Viejo, Y. Menendez-Menendez,

Figure 1. Overexpression of PD-1 decoy increases IFN-γ secretion from T cells. Activated B6 splenic T cells were transduced with retrovirus carrying either.

A All patients Arm A Arm B Deaths/patients 21/30 7/10 14/20

Tumor cells are dislodged into the pulmonary vein during lobectomy

Erin F. Wolff, M. D. , Naoya Uchida, M. D. , Ph. D. , Robert E

Notch signaling induces cytoplasmic CD3ϵ expression in human differentiating NK cells by Magda De Smedt, Tom Taghon, Inge Van de Walle, Greet De Smet,

Volume 9, Issue 5, Pages (November 1998)

CD56+/CD16− Natural Killer cells expressing the inflammatory protease granzyme A are enriched in synovial fluid from patients with osteoarthritis P.

The CXC-chemokine platelet factor 4 promotes monocyte survival and induces monocyte differentiation into macrophages by Barbara Scheuerer, Martin Ernst,

CD154 Expression Is Associated with Neutralizing Antibody Titer Levels Postinfluenza Vaccination in Stem Cell Transplant Patients and Healthy Adults

Lineages of human T-cell clones, including T helper 17/T helper 1 cells, isolated at different stages of anti–factor VIII immune responses by Ruth A. Ettinger,

Long-term expansion of effector/memory Vδ2− γδ T cells is a specific blood signature of CMV infection by Vincent Pitard, David Roumanes, Xavier Lafarge,

Yumi Matsuzaki, Kentaro Kinjo, Richard C Mulligan, Hideyuki Okano

Jacob Andrade, Shundi Ge, Goar Symbatyan, Michael S. Rosol, Arthur J

Fas-mediated apoptosis is important in regulating cell replication and death in trisomy 8 hematopoietic cells but not in cells with other cytogenetic abnormalities.

Expansion of FOXP3high regulatory T cells by human dendritic cells (DCs) in vitro and after injection of cytokine-matured DCs in myeloma patients by Devi.

FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization Sophia Björkander, Maria.

Michael H. Land, MD, Maria I. Garcia-Lloret, MD, Michael S

CD34+ Cells in the Peripheral Blood Transport Herpes Simplex Virus DNA Fragments to the Skin of Patients with Erythema Multiforme (HAEM) Fumitake Ono,

CD11c+ dendritic cells and plasmacytoid DCs are activated by human cytomegalovirus and retain efficient T cell-stimulatory capability upon infection by.

Frederic Geissmann, Steffen Jung, Dan R. Littman Immunity

Proportional change of CD4+CD25+ regulatory T cells after lymphocyte therapy in unexplained recurrent spontaneous abortion patients Hui Yang, M.D., Ph.D,

IL-21 inhibits T cell IL-2 production and impairs Treg homeostasis

CD56+/CD16− Natural Killer cells expressing the inflammatory protease granzyme A are enriched in synovial fluid from patients with osteoarthritis P.

Gentaro Izumi, Ph. D. , M. D. , Kaori Koga, Ph. D. , M. D

Jacob Andrade, Shundi Ge, Goar Symbatyan, Michael S. Rosol, Arthur J

Figure 3 Decreased AHI1 in human CD4+ T cells is associated with decreased proliferation and increased IFNγ production Decreased AHI1 in human CD4+ T cells.

Cysteinyl leukotriene E4 activates human group 2 innate lymphoid cells and enhances the effect of prostaglandin D2 and epithelial cytokines Maryam Salimi,

Reconstitution of Natural Killer Cells in HLA-Matched HSCT after Reduced-Intensity Conditioning: Impact on Clinical Outcome Caroline Pical-Izard, Roberto.

Jovana Maric, MSc, Avinash Ravindran, MSc, Luca Mazzurana, MSc, Åsa K

by Hairui Su, Chiao-Wang Sun, Szu-Mam Liu, Xin He, Hao Hu, Kevin M

Volume 140, Issue 1, Pages e3 (January 2011)

Transmembrane activator and CAML interactor (TACI) haploinsufficiency results in B- cell dysfunction in patients with Smith-Magenis syndrome Javier Chinen,

Prominent role of IFN-γ in patients with aspirin-exacerbated respiratory disease John W. Steinke, PhD, Lixia Liu, MD, Phillip Huyett, MD, Julie Negri,

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Steroid resistance of airway type 2 innate lymphoid cells from patients with severe asthma: The role of thymic stromal lymphopoietin Sucai Liu, PhD,

Targeting PKC in Human T Cells Using Sotrastaurin (AEB071) Preserves Regulatory T Cells and Prevents IL-17 Production Xuehui He, Hans J.P.M. Koenen,

Mark Gorelik, MD, Satya D. Narisety, MD, Anthony L

Katherine G. MacDonald, BSc, Nicholas A. J

Volume 18, Issue 3, Pages (March 2003)

Immunophenotyping of lymphocyte subsets.

LRBA protein expression in lymphocytes from a healthy individual and a patient. LRBA protein expression in lymphocytes from a healthy individual and a.

Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells.

Volume 28, Issue 6, Pages (June 2008)

DOCK8 protein expression in lymphocytes from a healthy individual.

Functional Assessment of Precursors from Murine Bone Marrow Suggests a Sequence of Early B Lineage Differentiation Events Kim-Sue R.S Tudor, Kimberly.

T-bet inhibits innate lymphoid cell–mediated eosinophilic airway inflammation by suppressing IL-9 production Ayako Matsuki, MD, Hiroaki Takatori, MD,

Impact of ONX 0914 on abundance and dissemination of monocytes/macrophages during CVB3 infection Impact of ONX 0914 on abundance and dissemination of monocytes/macrophages.

Binding competition between B2′B3 and B3 fragments of TcdB.

The B2′B3 region confers the binding phenotype of full-length TcdB.

Intrathymic T Cell Development and Selection Proceeds Normally in the Absence of Glucocorticoid Receptor Signaling Jared F Purton, Richard L Boyd, Timothy.

In Vivo Expansion of Regulatory T cells With IL-2/IL-2 mAb Complexes Prevents Anti- factor VIII Immune Responses in Hemophilia A Mice Treated With Factor.

Impact of ONX 0914‐induced neutrophilia during CVB3 infection

Thomas M. Schmitt, Juan Carlos Zúñiga-Pflücker Immunity

FGF/ERK inhibition accelerates neural fate conversion of EpiSCs.

Bone marrow transplantation for MHC class I deficiency corrects T-cell immunity but dissociates natural killer cell repertoire formation from function

Functional Analysis of Ionic Flux in Isolated Endosomes

Figure 1. Effects of Gal-4 on apoptosis and proliferation of monocytes

Increase in proliferation and activation of immune cells in peripheral blood after NKTR-214 treatment. Increase in proliferation and activation of immune.

Effect of nivolumab or ipilimumab on immune responses to vaccination in cynomolgus monkeys. Effect of nivolumab or ipilimumab on immune responses to vaccination.

S.varasteh 10/11/2012.

Fig. 2. Effects of CB_36 and its analogs in HepG2 cells

Surface expression of chemokine receptors on M

Presentation transcript:

Protective Immunity Project Chris Larsen, MD, DPhil, PI Christine Martens, Ph.D. Project Manager Vicki Hertzberg, Ph.D. Director of Data Management and Analysis Core

3 studies 2 human –New renal transplants (n=60) and 20 controls, followed for 2 years –Existing renal transplants (n=60) and 30 controls, receiving flu vaccine, followed for short time 1 macaque study, 30 macaques randomized to 6 groups

Longitudinal studies IDTime 1Time 2…Time t 1 … n

Measurements At each time point for each subject obtain measurements from: –Flow cytometry –ELISPOT/ELISA –M-array

Flow cytometry For each subject at each time, take several blood samples. For each blood sample, label all of the PBMCs with 8-10 markers (a panel). Cells will fluoresce according to the amount of each marker present. Apply 6-8 panels for each subject at each time. Each panel usually has 2 markers that are common to all other panels

What to the data look like? X-Y graph for fluoresence intensity for marker X vs marker Y. Gates are drawn that –Separate present from absent –Isolate cells of different types

Issues? How to analyze? Can we deal with fluorescence intensity rather than presence or absence? How do we institute QC for the gating?