Biology 22 Molecular Laboratory Report 1. Bacterial Transformation 2. Plasmid Isolation 3. RFLP Analysis RESULTS ONLY due on April 21 st, 2011 Discussion will be April 22 nd in class

Guidelines for the Results  Different science courses and scientific journals use varied approaches for the presentation of experimental Results but this molecular laboratory report will have only the presentation of the data but not a discussion of the data within the Results section.  The next few slides provide guidelines for preparing and submitting your Results.  The legends are not where data are discussed; simply present what the table or figure presents.  Again, the Result section is not the Discussion and must not include a discussion of the results.  Onto the specifics of the Results…

Guidelines for the Results  Include a Title Page  Provide a descriptive title  Your name  Must include group numbers (not individual lab partners) for each experiment  Results  All tables have titles and numbers above table  All figures have legends and numbers below figure  (Discussion: in lab on April 22 nd

What your Result Section must include: A Table that includes the Transformation results. The Table should be numbered and have a Title. The Title goes above the Table. The Calculation of your Transformation efficiency –If you did not obtain any transformation; this should be mentioned in your discussion; however since everyone observed results from a group who did have results from this experiment it is acceptable to use this for your transformation efficiency. A Photograph with a legend or caption of the isolated plasmid. The caption and figure number both are placed below the figure. A Photograph with a legend or caption of the RFLP. The legend or caption should be numbered and go beneath the figure. A graph of the Standard Curve with a legend or caption. A Table (with title) that summarizes the sizes and distances migrated for the ladder A Table (with title) that summarizes the sizes and distances migrated of the plasmid fragments from each appropriate lane Individual Maps for each restriction digest. Consider these maps collectively as a single figure so include a caption with this figure. Maps are not made for the ladder or for the uncut plasmid

Results: Transformation Table: results of 3 agar plates: number and color of colonies Calculation: of the Transformation efficiency

Results: plasmid isolation  The photograph of the plasmid isolation.  You should include a numbered legend or caption to this photograph. The legend includes: what samples are present on the gel, their “lane” number, and the type and percent gel.  Be sure and number the lanes of the gel.  Note: The gels were a 1.5% agarose

Results  A photograph of the RFLP analysis with figure legend or caption and number; the legend should be placed beneath the photograph.  Be sure and number the lanes of the gel.

Results  Standard curve Distance migrated (cm) DNA bp

Results  A separate table that shows the size (base pairs) of fragments versus distance migrated of your ladder (known) fragments. You may not be able to identify all 9 fragments of your ladder but you should be able to identify approximately 5 to 6 (or more) of these fragments.  A table that shows the size (base pairs) versus distance migrated of your unknown fragments

Provide MAP Results maps for each of the restriction digests.

Examples of MAP Results  These are just examples!  You will have third map with both enzymes and various sizes shown. Total Size: 7000 bp Lane 3 Alu1 Lane 4 Total size 7050 bp Hae

Number of Maps Lane 3 Lane 4 Lane 5 Map 1 Map 2 Map 3

A Discussion and data interpretation will be “in-class”.

The End