Diffusion

Selectively Permeable Membrane

Animal Cell (Normal Saline 0.9%) = solute

Diffusion experiment Methylene blue MW 320g/mole Potassium permagnate MW 150 g/mole Form Hypothesis T 0 initial diameter T 60 final diameter Activity 1 Room temp Calc. rate of diffusion

TimeMB RTPP RT T010 mm T6020 mm 10 mm/hr Table 1. Rate of Diffusion in Methylene Blue vs Potassium Permagnate at Room Temp

Table 2. Rate of Diffusion in Methylene Blue vs Potassium Permagnate at Room Temp Group Data GroupMB RTPP RT 110mm/hr8 mm/hr Ave SD

Create 4 dialysis bags, filled with unknown concentration sucrose solutions (10 ml) Weigh bags and then immerse in 10% sucrose solution (50 ml) Reweigh bags after 30 minutes of experiment Identify the bags containing 0%,10%, 20%, and 40% of sucrose, based on weight changes. Activity 2 Osmosis through semipermeable membrane

Unknown solutions of sucrose (0, 10, 20 & 40 %)

Dialysis Tubing 1.Open tubing with water 2.Tie knot at one end 3.Fill with one of the unknown solutions (10ml) 4.Tie other end

Weigh pre and post

50 ml 10% sucrose Unknown solution (10ml) (0, 10, 20 & 40 % sucrose solution) 30 minutes