INBRE Science Research Core Purposes: 1) to provide statewide access to state-of- the-art research technology not commonly found at PUIs 2) to offer networking through an instate Annual Scientific Research Symposium

INBRE Support of Facilities The Arkansas INBRE supports existing core facilities on the UAMS and UAF campuses Facilities are chosen primarily on the basis of Project Leader needs INBRE support to each facility -Percentage of personnel (director and technician’s salaries) -Some equipment purchase and/or service contracts -Supply budget No actual fees for INBRE researchers – but only minimal support for supplies

Science Research Core – R. Koeppe, PhD, UArk Core Director FACILITIES: Genomics Facility Helen Beneš, PhD Proteomics Facility Alan Tackett, PhD Joshua Sakon, PhD Digital Microscopy Facility Richard Kurten, PhD

Services Offered:  Plasmid DNA preparation  Recombinant plasmid construction  Subcloning of PCR products or genomic DNA fragments  Generation of reporter gene constructs  Addition of fluorescent or antigen-specific tags  Construct verification with help of the UAMS DNA sequencing facility  In vitro Mutagenesis  Construct verification by DNA sequencing Genomics Facility – H. Beneš, PhD, UAMS, Director

Proteomics Facility – A. Tackett, PhD, UAMS and J. Sakon, PhD, UArk, Directors Facility grew out of first Arkansas COBRE award. Services Offered:  Protein characterization by mass spectrometry  Identification of unknown proteins  Quantitative comparison of proteins in biological/medical samples  Mapping of post-translational modifications  Access to specialized instrumentation  LTQ XL ion trap, MALDI-prOTOF and MALDI-LTQ ion trap mass spectrometers  ProGest robot for “in-gel” digestion of polypeptides  Mascot server for data analysis

Digital Microscopy Facility – R. Kurten, PhD, UAMS, Director Services Offered:  Access to multiple microscope workstations  Especially for static and time-lapse imaging of living cells, using controlled perfusion systems  With microinjection, FRET and FRAP capabilities  For imaging of fluorescently labeled fusion proteins  For imaging of fixed biological/medical samples  Training of researchers to collect multicolor images by fluorescence microscopy

Facility Workshops  Presented by Facility Directors in different venues - Lunchtime presentations to Student Summer Fellows - Broadcast through Access Grid studios to UArk and UALR - Workshops at Annual Research Symposium - Small group training sessions as requested

Facilities Selected for INBRE Renewal Table 11.Projected INBRE Facility Usage by Project Leaders (see individual proposals for details) Project Leader Digital Microscopy Proteomics DNA Damage & Toxicology Beckford X X Benjamin X Caro X Dearolf X Hensley X Hill X Huang X Kelley X Lorence X Naylor X X Perry X Rice X X Wangila X X