DNA and RNA The Molecule of Life: DNA and RNA
DNA vs. RNA Summary DNARNA By comparison they both have: Sugar phosphate background Nitrogenous bases By contrast each has: Double strand, double helix Single strand and various shapes (3) Deoxyribose sugarRibose sugar A=T and C=GA=U and C=G Stay in the nucleusNucleus and cytoplasm
Different 5 – C sugar: Deoxyribose (DNA) and Ribose (RNA)
Thymine (DNA) and Uracil (RNA) Thymine versus Uracil Thymine in DNA is replaced by Uracil in RNA
vs DNA RNA
Ribonucleic Acid Types (p ) There are several types. We will focus on the main 3 types: rRNA: large, makes up structure of ribosomes. - Large globular structure, forms structure with proteins to form ribosome tRNA: smaller, contains amino acid to match code of mRNA. Compact 3-D structure mRNA: single strand, provides complementary code from DNA for protein - Single strand may fold back on itself to form H-bonds
rRNA rRNA: Ribosomal ribonucleic acid Located in ribosomes Provides the site on the ribosome where the protein is assembled
rRNA Structure This big “globular” structure is the structure for the ribosome all spread out. A smaller “glob” provides the second part of the ribosome structure.
Ribosome: rRNA
tRNA tRNA: Transfer ribonucleic acid In humans, more than 40 different tRNA float freely in the cytoplasm, float to ribosome for protein synthesis Each tRNA is connected to a different amino acid Function: to place the correct amino acid in a protein sequence
tRNA Structure Specific regions: 3’ end is AMINO ACID site Anticodon is complementary to mRNA codon (code)
tRNA detail Typically 75 nucleotides long, twisted into a tertiary structure (3-D) Specific in function to translation of mRNA into protein amino acid sequencing
tRNA detail Anticodon: three nucleotide sequence that correlates to a specific amino acid that is attached to its 3’end (amazing isn’t it!) Anticodon complements the mRNA three nucleotide sequence called the codon.
mRNA mRNA: Messenger ribonucleic acid Long single strand of RNA that has the coded complement from the DNA. Can fold back to form H-bonds with itself Brings the code from the nucleus to the cytoplasm, specifically to ribosomes.
mRNA from DNA – Transcription DNA molecule is opened at a specific gene site mRNA is assembled complementary to one DNA strand When coding is complete, it detaches and floats to the ribosome DNA re-fits itself. Key enzyme: RNA Polymerase
mRNA formed at TRANSCRIPTION
Summary: 3 Main RNA Forms