Circulating Cell-Free DNA Necrosis, apoptosis, and possibly “active secretion” are thought to be the mechanisms by which cell-free nucleic acids enter the circulation. 0.18Kb Amos Douvdevani, PhD Department of Clinical Biochemistry and Pharmacology, Soroka Medical Center & Ben-Gurion University of the Negev
Dilution of sera in buffer containing SYBR Gold stain Measurement of fluorescence Direct fluorometric Method for DNA Measurement Goldshtein et al. Ann Clin Biochem 2009; 46: 488–494 Mix & Measure, immediate quantitative results Cost of reagents $5/100 samples Circulating free DNA Could be tested in the clinic using a small device (DNAmeter) SYBR Gold stain Serum/ plasma 20 l
Few ideas for Possible collaboration Diagnosis of pneumonia (reduce X-ray) Diagnosis of flue and other viral diseases Early cancer detection Any idea you have (free assay for all) Amos Douvdevani, Tel: