B C Supplementary Figure 1 Control X0 Control X9 -HBx (mAb) -HBx (rabbit serum) ABCEFD aa ABCEF X0 X9 ( D) A Control X0 X9 Control X0 X9 Control X0 X9 Control X0 X9 Control X0 X9 Control X0 X9 Control X0 X9 Control X0 X9 Post Double Thymidine Block (h) Cells (%)
Cyclin A Binding (68-108) DNA Binding ( ) DP-1 Binding ( ) Transactivation Domain ( ) Rb binding E2F1-WT E2F1 (1-374) Transactivation Domain mutant Wild-type C B Normalized luciferase units Control X0 pGL3 CDC6 WT CDC6-SM1 # # A E2F1 CCAAT NF AP CDC6 WT Wild type E2F1 binding site TTTGGCGC E2F1 Mutant E2F1 binding site TTTAACGC CDC6-SM1 E2F Luciferase CDC6 promoter [-1534, +225] Supplementary Figure 2
E2F E2F pGL3-E2F1 WT pGL3-E2F1 Mut Luciferase E2F1 CCAAT Sp CCAAT Sp E2F1 promoter [-728, +127] Supplementary Figure 3
Relative abundance of CDC6 on human -globin origin of replication (arbitrary unit) Serum Stimulation (h) Relative abundance of CDC6 on human -globulin locus (arbitrary unit) Serum Stimulation (h) ChIP -CDC6 Control Input Serum Stimulation Control X0 X9 A 3h 7h Control ChIP Input Serum Stimulation Control X0 X9 B 3h 7h -CDC6 Human -globin origin -globulin non-origin locus Supplementary Figure 4