MCB 186 CIRCADIAN BIOLOGY Clock genes & Biochemical Mechanisms: Kai genes October 10, 2007 J. W. Hastings

CORE CLOCK COMPONENTS IN FEEDBACK LOOPS OF 3 SYSTEMS

TEMPORAL SEPARATION OF CELL BIOCHEMICAL ACTIVITIES IN CYANOBACTERIA ( OXYGEN, NITROGENASE ETC) MITSUI ET AL 1986

CIRCADIAN RHYTHM of CYANOBACTERIAL GENE mRNA Kondo et al PNAS LUCIFERASE REPORTER

Turntable Screening Apparatus: 12 positions for petri dishes or titer plates

BACTERIAL COLONIES EXPRESSING BIOLUMINESCENCE Kondo et al PNAS

Cyanobacterial in vivo Rhythm with Bacterial Luciferase as a Reporter Luminescence Kondo, Johnson Golden et al., 1993, PNAS

EFFECT OF DARK EXPOSURES ON PERIOD In LL during ---- In light but with 3 min in dark every 30 min

DARK PULSE PHASE SHIFTS IN CYANOBACTERIA KONDO ET AL 1993

TEMPERATURE has SMALL EFFECT on PERIOD of BACTERIAL RHYTHM

PERIOD MUTANTS OF CYANOBACTERIA KONDO ET AL.1995

CYANOBACTERIA ARHYTHYMIC & AMPLITUDE MUTANTS KONDO 1995

PERIOD VARIATION OF INDIVIDUAL CYANOBACTERIA MUTANT CLONES KONDO ET AL.1995

LUCIFERASE REPORTER RANDOMLY INSERTED IN FRONT OF PROMOTERS IN BACTERIA. ABOUT 800 LUMINOUS COLONIES ISOLATED ALL EXHIBITED CIRCADIAN RHYTHMS ACROPHASES NOT ALL THE SAME

ACROPHASE CLASSES OF CLOCK-CONTROLLED GENES LIU ET AL,1995

KAI genes in SYNECHOCOCCUS and MUTANT MAPPING ISHIUR

PHENOTYPES OF KAI GENE INACTIVATIONS ISHIURA ET AL 1998

EXPRESSION OF KAI GENE CONSTRUCTS ISHIURA ET AL 1998

Cyanobacterial Clockworks TTO Model Ishiura et al., 1998

Cyanobacterial Clockwork Model TRANSCRIPTIONAL CONTROL OF MANY GENES 2003 Xu et al., EMBO J. 22: , 2003

Kai C PHOSPHORYLATION RHYTHM in vitro in a SOLUTION CONTAINING only ATP with Kai A, B and C Phase is not determined by light Nakajima et al, Science, 308: (2005 )

IN VITRO CIRCADIAN RHYTHM KaiC PHOSPHORYLATION

In vitro phosphorylation rhythm is temperature compensated Nakajima, M et al Kondo, T (2005) Reconstitution of circadian oscillation in vitro Science 308:

In vitro phosphorylation rhythm is temperature compensated Nakajima, M et al Kondo, T (2005) Reconstitution of circadian oscillation in vitro Science 308:

Period of phosphorylation rhythm corresponds to mutant period Tomita, Nakajima, Kondo & Iwasaki (2005) Science 307:

Tau of in vitro P-oscillation corresponds to mutant tau Nakajima, M et al Kondo,T (2005) Reconstitution of circadian oscillation in vitro Science 308:

Simplified model for the posttranslational oscillator Nakajima, M et al Kondo,T (2005) Science 308:

EVIDENCE THAT TRANSCRIPTION and TRANSLATION are not NECESSARY for CIRCADIAN OSCILLATIONS IN CYANOBACTERIA Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedback in circadian rhythm of KaiC phosphorylation Science 307:

NO RHYTHM OF kaiA or kaiBC mRNAs in DD Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedback in circadian rhythm of KaiC phosphorylation Science 307:

NO RHYTHM OF KaiC PROTEIN in DD Tomita, Nakajima, Kondo & Iwasaki (2005) Science 307:

RHYTHM OF KaiC PROTEIN PHOSPHORYLATION in DD Tomita, Nakajima, Kondo & Iwasaki (2005) Science 307:

Period Temperature Compensation of in vivo Rhythm of KaiC Phosphorylation in DD Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedback in circadian rhythm of KaiC phosphorylation Science 307:

Model for the posttranslational oscillator coupled with TTO Tomita, Nakajima, Kondo & Iwasaki (2005) No transcription-translation feedbackin circadian rhythm of KaiC phosphorylation Science 307:

Tau of in vitro P-oscillation corresponds to mutant tau Nakajima, M et al Kondo, T (2005) Reconstitution of circadian oscillation in vitro Science 308: