Lab. Safety, Microscopes and Colony Morphology Supplemental instruction Picture from

Slides:



Advertisements
Similar presentations
Biology Microbiology Kathleen Devlin
Advertisements

Experiment one Basic methods of microbiological laboratory Basic methods of microbiological laboratory  Bacterial culture  Staining of bacteria  Use.
Using the Microscope Laboratory 1 Biology 170. What did the invention of the microscope enable? To study what organisms are made of Individual cells could.
Introduction to the Microscope
Lab 11& 12 Biochemical Characterization Practice questions Designed by Pyeongsug Kim ©2010 Fall 2011 Chattahoochee.
Bacteria are Everywhere By: Lauren Senter Dr. Hamrick STEP Program at Campbell University.
Crystal Gram’s (Primary stain) Gram’s (Primary stain) Gram’s Crystal violet Gram’s iodine Safranin Alcohol.
FIRST ASSIGNMENT: Clear desks completely.
Microbiology.
Introduction to the oil immersion compound microscope
Studying the Microbial World (microscopes) Supplemental instruction Designed by Pyeongsug Kim ©2010 Picture from
COLONY MORPHOLOGY ON AGAR PLATE CULTURES
226 PHT Lab#2 Staining techniques
Introduction to Microscopy. Objectives Learn to use a compound microscope correctly. Diagram the path of light through a compound microscope. Name major.
Microbiology. The study of organisms too small to be seen without magnification. –Bacteria –Viruses –Fungi –Protozoa –Helminths (worms) –algae.
Warm-Up 1/14/09 List your classes and what kind of a final you are having in that class. Turn in warm-ups today.
1) Ocular Lens/Eyepiece Magnifies the specimen image 10x 2) Nose piece The Nose Piece holds the objective lenses and can be turned to increase.
Microscopy Compound light microscope is composed of: 1- stand 2- stage 3- substage ( condenser, diaphragm) 4- body tube (carrying lens system) 5- light.
Study Guide to Help with Laboratory Exam 1 Microbiology 1214 A partial study guide only. Not intended to replace the review sheets or the “Know the following”
Introduction to bacteria: Bacteriology Lecture 4 13/9/2015.
2A: Microscopy. Post Lab 2 is assigned today and due by the time your lab meets next. Pre Lab 3 will be available on Wednesday at 5 PM and is also due.
Class 2 Review of Class One - Microscopy and Introduction to Aseptic Technique and Media Mrs. Sidelsky.
Biochemical Characterization
Classification of bacteria
أ. شروق الشهراني بسم الله الرحمن الرحيم 140 MICRO LAB 6: SIMPLE STAIN + N EGATIVE S TAIN.
General Comments about the Microbial World Supplemental instruction Designed by Pyeongsug Kim ©2010 Picture from
Negative Stain and Acid Fast Stain
Supplemental instruction Chattahoochee Technical College
One way to classify bacteria is by examining its morphology. Bacteria appear in many different shapes. We will examine the three basic shapes but note.
Laboratory Tips and Microscope Use. Setting Up: Microscope Carrying and Bench Layout Always hold the arm of the microscope while supporting the base with.
BACTERIA Unicellular Organisms Prokaryotes (no true nucleus)
Cornel Style notes in your spiral notebook!. Microbiology The study of microorganisms, which are tiny organisms that live around us and inside our body.
313 PHT LAB#1 ☠ Lab coat & marker. ☠ No eating, drinking, ☠ Benches disinfection. ☠ Inoculating loop sterilization. ☠ Aseptic technique. ☠ Discarded.
SCIENCE TOOLS Read each slide. Look at each picture. Answer each question.
Lab Exercise 4: Simple Staining and Bacterial Cell Morphology.
Simple Staining and Bacterial Cell Morphology
Lab 3 Goals and Objectives: Exercise 7: Ubiquity of Bacteria: Observe results Exercise 9: Aseptic Technique: Check results Exercise 10: Pure Culture Technique.
Simple stain Rana A.Abo-Hajjras. Simple stain The simple stain can be used to determine cell shape, size, and arrangement. True to its name, the simple.
Introduction to Lab Ex. 11:
Welcome to Microbiology Lab Tue 6:00-9:10pm Instructor: Huma Malik Office location: MTSC 339 Office hours: Tue 5:00-6:00pm (or.
1. 2 Microbiology is the science that deals with the study of too small organism (micro-organisms) that are invisible to the naked eye.
Plate 11 Bacterial Shapes. Bacillus Bacillus = rod µm in length.
2. Eubacteria “true” bacteria microscopic
Important Lab instrument
Lab #2.
Focusing the microscope simple staining
SAFETY IN THE LAB. 1.Stay in your place. Never run or play in the laboratory.
MICROBIOLOGY LAB 4 BIDISHA PAUL 261BH.
Lab 3 Goals and Objectives :
WHAT IS A CELL? (pro vs. eu) CHALLENGE: What are the similarities and differences between PROKARYOTIC and EUKARYOTIC cells?
2 pt 3 pt 4 pt 5pt 1 pt 2 pt 3 pt 4 pt 5 pt 1 pt 2pt 3 pt 4pt 5 pt 1pt 2pt 3 pt 4 pt 5 pt 1 pt 2 pt 3 pt 4pt 5 pt 1pt Identifying organisms Part 1 Identifying.
1. 2 Microbiology is the science that deals with the study of too small organism (micro-organisms) that are invisible to the naked eye.
Microbiology Laboratory Procedures.  Microbiology = the study of microbes (bacteria, fungi, and viruses) Bacteriology = study of bacteria Virology =
أ. شروق الشهراني بسم الله الرحمن الرحيم 140 MICRO LAB 6: S IMPLE S TAIN + N EGATIVE S TAIN.
Lab 1 – Measurement and Microscopy. Welcome to Lab!  Be sure to familiarize yourself with safety procedures ○ Where does broken glass go? ○ What goes.
Chap. 4 : Bacteria I. Characteristics of Bacteria 1. Kingdom : Monera 2. Unicellular (1 celled), prokaryote can fit inside a period 4. Found everywhere.
Bacteria Kingdoms Archaebacteria and Eubacteria. Bacteria are known in two types: Ancient Bacteria – Archaebacteria True Bacteria – Eubacteria They are.
Lab 1 – Part 1: Media Types and Uses
COOLING CURVE OF PALMITIC ACID LAB
How We Study Science Tools/ Equipment.
WASTE DISPOSAL FLOWCHART
COOLING CURVE OF PALMITIC ACID LAB
How does one calculate total magnification on your microscope?
Dr. Souki MICRO 200 Cerritos College
Introduction to Science
140mic General Microbiology
Simple Staining and Bacterial Cell Morphology
Pg Beaker.
Media.
COLONY MORPHOLOGY ON AGAR PLATE CULTURES
Presentation transcript:

Lab. Safety, Microscopes and Colony Morphology Supplemental instruction Picture from Designed by Pyeongsug Kim ©2010 Fall 2011 Chattahoochee Technical College

Designed by Pyeongsug Kim, © Picture from Lab. Safety Food and Drink Not allowed. Safety goggles, Please…. Dispose materials in proper place.

Designed by Pyeongsug Kim, © Dispose materials solid Bacteria containing solid materials. To biohazard can. Microscopic slides To glass disposal box Picture from liquid Bacteria containing liquid test tubes. To waste rack

Designed by Pyeongsug Kim, © Picture from Dispose agar plates In order to dispose agar plate, wrap the plate with lab. film before putting the biohazard can.

What type of microscope are you seeing? Light compound microscope In which object do you need immersion oil to see the bacterial sample? 100X objective Why do you need oil-immersion? (how the oil-immersion work?) Bacteria is too small; Oil – to increase the magnification and to remove light refraction and to improve resolution. Designed by Pyeongsug Kim, ©2010

D C A B What are the names of these parts? Write each function. E The answer is in your textbook or lab. notebook. Designed by Pyeongsug Kim, ©2010

10 X 40 X 100X 40 X 10 X 100X Total magnification on the slide in view? You want to see in 600 times magnification. Which part do you change to? (turn into..) 100x 400x 60x objective Designed by Pyeongsug Kim, ©2010

Designed by Pyeongsug Kim, © Colony Morphology Picture from

Colony Morphology and Arrangements Designed by Pyeongsug Kim, © Cocci DiplococcusDiplococcus: cocci arranged in pairs. StreptococcusStreptococcus: cocci arranged in chains Staphylococcus:Staphylococcus: cocci arranged in irregular, often grape-like clusters Diplococcus Picture from Staphylococcus Streptococcus

Colony Morphology and Arrangements Designed by Pyeongsug Kim, © Bacillus(Rods) BacillusBacillus: a single bacillus DiplobacillusDiplobacillus: Bacilli that remain in pairs after they divide. StreptobacillusStreptobacillus: bacilli in chains CoccobacillusCoccobacillus: oval and similar to a coccus Picture from Bacilli Streptobacilli

Designed by Pyeongsug Kim, © Picture from