Bacterial Transformation Teacher Preparation Sterile Materials

Sterile Materials Paper Clips, Glass Pipettes and Toothpicks Heat the foil packages containing paper clips, glass pipettes, and toothpicks at 350 º F for 30 minutes to sterilize. Bacterial Transformation Teacher Preparation

Sterile Materials 1.5 ml Tubes Sterilize ml microcentrifuge tubes for each group of students. Wrap the tubes in aluminum foil and heat at 250 º F for 30 minutes. Bacterial Transformation Teacher Preparation

Sterile Materials Distilled Water In a bottle with a cap, add 50 ml of distilled water. Cap the bottle and place it in boiling water for 30 minutes. Refrigerate to prevent contamination. Bacterial Transformation Teacher Preparation

Sterile Materials CaCl 2 Solution Step 1 Dissolve 0.75 g of CaCl 2 into 50 ml of distilled water in a labeled 100-ml glass bottle with a cap. Bacterial Transformation Teacher Preparation

Sterile Materials CaCl 2 Solution Step 2 Cap the bottle and place it in boiling water for 30 minutes. Refrigerate to prevent contamination. Bacterial Transformation Teacher Preparation

Sterile Materials Ampicillin Solution Add 1 ml of sterile distilled water to a 50 mg tube of ampicillin powder (provided to Iowa teachers by the Office of Biotechnology). Bacterial Transformation Teacher Preparation