DNA / RNA. tRNA rRNA mRNA 5’ 3’ Types of RNA Transcription: transfer of information from DNA to RNA in the nucleus  In the process of transcription.

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Presentation transcript:

DNA / RNA

tRNA rRNA mRNA 5’ 3’ Types of RNA

Transcription: transfer of information from DNA to RNA in the nucleus  In the process of transcription DNA is used to make a complimentary strand of RNA  The WHOLE strand of DNA doesn’t get turned into RNA at once  Instead, as needed, small sections of DNA are transcribed into RNA Sections are called???  GENES

Transcription: If a gene is transcribed into mRNA it is being “expressed” Having the gene in your DNA is not enough It MUST be turned into RNA to do anything

Genetic Code: Every three bases of the mRNA is called a Codon Each codon represents an Amino Acid The first codon of a gene is ALWAYS AUG 5’ 3’ AU GA GG

Genetic Code: There are 64 possible three base combinations of AUGC 1 is the start codon AUG (methionine) 3 are stop codons and tell the cell when the protein is done: UAA, UAG, UGA The other 60 represent the other 19 amino acids.

Genetic Code: AUG tells the cell to bring in the amino acid Methionine AGG tells the cell to bring in a different amino acid, arginine 5’ 3’ AU GA GG

Translation: The mRNA formed in transcription is taken to the ribosome where it serves as the directions to form a sequence of amino acids which form proteins. - Ribosome is made out of protein and rRNA

Translation: Step 1: mRNA lines up in the ribosome 5’ 3’ AU GA GG

Translation: Step 2: Enter tRNA - tRNA is a twisted up piece of RNA with an amino acid attached to one end

V. Translation: Step 2: Enter tRNA - Only bases we are interested in are the 3 at the bottom - These are called the Anti-codon

Translation: Step 2: Enter tRNA - There is a tRNA with a unique anti- codon for each Amino Acid - The anti-codon binds to its complimentary codon in the mRNA

Translation: 5’ 3’ Step 2: tRNA with anti-codon complimentary to AUG enters Ribosome with the first amino acid AA 1

Translation: AA 2 5’ 3’ Step 3: Next tRNA enters and chain of amino acids begins AA 1

Translation: 5’ 3’ Step 3: Next tRNA enters and chain of amino acids begins AA 1 AA 2

Translation: 5’ 3’ Step 3: Next tRNA enters and chain of amino acids begins AA 1 AA 2

Translation: 5’ 3’ Step 4: A covalent Bond forms between AA1 and AA2 AA 1 AA 2

Translation: 5’ 3’ Step 5: Ribosome shifts, releases tRNA 1, opens up new codon on the mRNA AA 1 AA 2 AA 3

Translation: 5’ 3’ Step 5: Ribosome shifts, releases tRNA 1, opens up new codon on the mRNA AA 1 AA 2 AA 3

Translation: 5’ 3’ Step 5: Ribosome shifts, releases tRNA 1, opens up new codon on the mRNA AA 1 AA 2 AA 3

Translation: 5’ 3’ Step 5: Ribosome shifts, releases tRNA 1, opens up new codon on the mRNA AA 1 AA 2 AA 3

Translation: 5’ 3’ Step 6: Process continues until open codon is one of the 3 STOPS. AA 1 AA 2 AA 3 STOP

VI. Translation: 5’ 3’ Step 7: Completed Protein is released. AA 1 AA 2 AA 3 STOP

Transcription (happens in the nucleus) Translation (happens at the ribosome)

Translate the mRNA an amino Acid sequence.

Useful links - Lew - Port's Biology Place