Supplementary Figure 1. Supplementary Figure 2 Suppl. Fig. 1. The promoter of miR-155 encoding Bic gene contains two putative NF-  B binding sites.

Slides:

Advertisements

Similar presentations

Exogenous B56γ3 WT F395C WT F395C IgG Vinc Endogenous B56γ2,3 HA-IPInput Figure S1 HA-B56γ3 is unable to interact with endogenous B56γ. Lysates of U2OS.

Advertisements

Basal Promoter Elements Basal Promoter Element = BPE TATA Box: (G or A)TATA(A or T)AA –Nature 381: (1996) –Science 272: (1996) CAAT Box:

Figure S1 Figure S1. Modular structure of rice proteins encompassing the SPX domain. Different colors indicate the amino acid ranges of the domains. Purple,

Transcription control elements (DNA sequences) are binding sites for transcription factors, proteins that regulate transcription from an associated.

Figure S1. Alignment of sequences from the 5′-end to the Sm binding site of reported genomic sequences (9-15) for HSUR 1. MicroRNA binding sites are.

Supplementary Figure 1 Supplementary Figure 1. Representative micrographs of migration and invasion assays. miR-452 or si-WWP1 transfectants inhibited.

Supplementary Figure 1. (A) Expression of DSB repair proteins

Volume 342, Issue 1, Pages (January 2014)

Volume 16, Issue 3, Pages (March 2008)

NF-κB Accumulation Associated with COL1A1 Transactivators Defects during Chronological Aging Represses Type I Collagen Expression through a –112/–61-bp.

Quiz#7 LC710 10/18/10 name___________

Volume 138, Issue 3, Pages e2 (March 2010)

Intermittent cyclic mechanical tension promotes endplate cartilage degeneration via canonical Wnt signaling pathway and E-cadherin/β-catenin complex cross-talk

Histone deacetylase inhibitors increase microRNA-146a expression and enhance negative regulation of interleukin-1β signaling in osteoarthritis fibroblast-like.

Substance P Enhances the Production of Interferon-induced Protein of 10 kDa by Human Keratinocytes in Synergy with Interferon-γ Naoko Kanda, Shinichi.

Volume 138, Issue 2, Pages (February 2010)

Suppl.Figure 1 Transfected HEK293 cells -130kDa -100KDa Blot: -V5

Volume 136, Issue 5, Pages (May 2009)

Measurement of fetal γ-globin expression due to KLF1 being a negative or positive regulator By: Ashria Arora.

IFN-γ Upregulates Expression of the Mouse Complement C1rA Gene in Keratinocytes via IFN-Regulatory Factor-1 Sung June Byun, Ik-Soo Jeon, Hyangkyu Lee,

A B C Vinculin Vinculin HBP1 HBP1 p63 Loricrin Supplementary 2.

Sp1 Is Required for Glucose-Induced Transcriptional Regulation of Mouse Vesicular Glutamate Transporter 2 Gene Tao Li, Liqun Bai, Jing Li, Suzu Igarashi,

Volume 10, Issue 1, Pages (July 2004)

PU.1/Spi-B Regulation of c-rel Is Essential for Mature B Cell Survival

Everolimus Inhibits SMC Proliferation Through Transcriptional Repression of Telomerase (A) Cell proliferation assay in primary murine vascular smooth muscle.

Superoxide enhances interleukin 1β–mediated transcription of the hepatocyte-inducible nitric oxide synthase gene Paul C. Kuo, Keith Abe, Rebecca A. Schroeder

17β-Estradiol Inhibits MCP-1 Production in Human Keratinocytes

Role of Sp1 in Transcription of Human ATP2A2 Gene in Keratinocytes

Fig. 5. GUCD1 was a direct target of miR-370 in HCC

Molecular Mechanisms Regulating the Defects in Fragile X Syndrome Neurons Derived from Human Pluripotent Stem Cells Tomer Halevy, Christian Czech, Nissim.

Volume 57, Issue 5, Pages (May 2000)

Volume 14, Issue 6, Pages (February 2016)

Histamine Enhances the Production of Granulocyte-Macrophage Colony-Stimulating Factor via Protein Kinase Cα and Extracellular Signal-Regulated Kinase.

Cyclooxygenase-2 Inhibitor Enhances Whereas Prostaglandin E2Inhibits the Production of Interferon-Induced Protein of 10 kDa in Epidermoid Carcinoma A431

KIF1Bβ promotes DHX9 nuclear localization.

17β-estradiol Inhibits the Production of RANTES in Human Keratinocytes

Volume 10, Issue 3, Pages (September 2006)

ERα directly binds to pri‐miR‐22

Volume 126, Issue 4, Pages (April 2004)

Both Natural and Designed Micro RNAs Can Inhibit the Expression of Cognate mRNAs When Expressed in Human Cells Yan Zeng, Eric J Wagner, Bryan R Cullen

MicroRNA Destabilization Enables Dynamic Regulation of the miR-16 Family in Response to Cell-Cycle Changes Olivia S. Rissland, Sue-Jean Hong, David P.

Volume 3, Issue 4, Pages (April 2003)

Nef Triggers a Transcriptional Program in T Cells Imitating Single-Signal T Cell Activation and Inducing HIV Virulence Mediators Alison Simmons, Varuna.

Identification of Rare, Disease-Associated Variants in the Promoter Region of the RNF114 Psoriasis Susceptibility Gene Alexandros Onoufriadis, Michael.

Volume 39, Issue 5, Pages (November 2003)

Volume 119, Issue 2, Pages (August 2000)

Validation of phosphatase hits mapping prediction on the optimized growth model. Validation of phosphatase hits mapping prediction on the optimized growth.

SUMO-1 Modification Represses Sp3 Transcriptional Activation and Modulates Its Subnuclear Localization Sarah Ross, Jennifer L Best, Leonard I Zon, Grace.

Epigenetic Silencing of Lysyl Oxidase-Like-1 through DNA Hypermethylation in an Autosomal Recessive Cutis Laxa Case Romain Debret, Valérie Cenizo, Géraldine.

tssRNA promoter analysis and function.

Dan Yu, Rongdiao Liu, Geng Yang, Qiang Zhou Cell Reports

USP2a-dependent miRs deregulation modulates MYC expression.

Volume 25, Issue 10, Pages e8 (December 2018)

Negative Regulation of Tumor Suppressor p53 by MicroRNA miR-504

Lawrence M. Pfeffer, Andrzej T. Slominski

Volume 10, Issue 19, Pages (October 2000)

Defining the Regulatory Elements in the Proximal Promoter of ΔNp63 in Keratinocytes: Potential Roles for Sp1/Sp3, NF-Y, and p63 Rose-Anne Romano, Barbara.

The NF-κB-binding site of the uPAR promoter is required forβ 3-endonexin-mediated downregulation of the uPAR gene transcription. The NF-κB-binding site.

Enhanced Tailored MicroRNA Sponge Activity of RNA Pol II-Transcribed TuD Hairpins Relative to Ectopically Expressed ciRS7-Derived circRNAs Anne Kruse.

Synergistic WNT1 and WNT7B signaling is downstream of LRP6 phosphorylation and β-catenin stabilization. Synergistic WNT1 and WNT7B signaling is downstream.

Rb Interacts with Histone Deacetylase to Repress Transcription

NF-κB superrepressor was overexpressed by means of the bicistronic retrovirus. NF-κB superrepressor was overexpressed by means of the bicistronic retrovirus.

Association of CNK1 with Rho Guanine Nucleotide Exchange Factors Controls Signaling Specificity Downstream of Rho Aron B. Jaffe, Alan Hall, Anja Schmidt

Targeting DCLK1 by miRNA-137.

A Splicing-Independent Function of SF2/ASF in MicroRNA Processing

Fig. 3. KMS99220 inhibits activation of NFκB signaling via HO-1 induction. (A) BV2 cells were treated with 10 µM KMS99220 for 1 h, and then treated with.

Fig. 4 Dcr-2 binds to the 3′UTR of Toll mRNA.

The interaction of PALB2 with BRCA1 is required for the assembly of PALB2, BRCA2, and RAD51 nuclear foci. The interaction of PALB2 with BRCA1 is required.

Stéphane Karlen, Lasse R. Braathen

Transcriptional Regulation of the Elafin Gene in Human Keratinocytes

Presentation transcript:

Supplementary Figure 1

Supplementary Figure 2

Suppl. Fig. 1. The promoter of miR-155 encoding Bic gene contains two putative NF-  B binding sites. It is drown a partial nucleotide sequence of Bic promoter (from to – 222; from – 1064 to – 1912) containing the putative cis elements with higher score. The TATA box (-25 in pink), the AP1 site (-40 in red) the SP1 site (- 97 in blue), the two NF-  B binding sites (-1150; in green), and the PU.1 site ( in purple). Suppl Fig. 2. Inhibition of NF-  B canonical pathway leads to a reduction of miR-155 levels in EBV immortalized MC3 cells. A) Western Blot analysis of total protein extracts obtained from MC3 untransfected (MC3 UT), MC3 transfected with pRc/CMV-HA-I  B  -S32/36A (MC3 I  B  and MC3 transfected with Empty Vector (MC3 EV). The blot was analyzed for I  B  expression. The exogenous protein runs higher than the endgenous because has HA- FLAG. B) Western Blot analysis of nuclear extracts obtained from MC3 untransfected (MC3 UT), MC3 transfected with pRc/CMV-HA-I  B  -S32/36A (MC3 I  B  and MC3 transfected with Empty Vector (MC3 EV). The blot was analyzed for p65 expression and normalized by H3. C) Graphic representation of the Western blot showed in B results as average of three independent experiments. The nuclear p65 decreases when the undegradable form of I  B  is expressed. D) Northern Blot analysis of total RNAs obtained from MC3 untransfected (MC3 UT), MC3 transfected with pRc/CMV-HA-I  B  -S32/36A (MC3 I  B  and MC3 transfected with Empty Vector (MC3 EV). The blot was analyzed for miR-155 expression and normalized by U6. E) Graphic representation of the Northern blot results as average of three independent experiments.