11/2/20151 THE MICROSCOPE Use of the Microscope Lab Manual Exercise # 2.

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Presentation transcript:

11/2/20151 THE MICROSCOPE Use of the Microscope Lab Manual Exercise # 2

11/2/2015ALFONSO A. PINO MD.2 THE MICROSCOPE

11/2/20153 THE COMPOUND MICROSCOPE

11/2/20154 BASE It contains: Off on switch Voltage regulator illuminator

11/2/20155 STAGE It supports the microscope slides and contains slide clips and mechanical stage

11/2/20156 MECHANICAL STAGE It has 2 knobs for movement of the slides from left to right and front to back

11/2/20157 SUBSTANCE CONDENSOR It has 3 parts: blue filter iris diaphragm condenser lens

11/2/20158 Blue filter: the short wavelength of the blue light provides maximum resolution Iris diaphragm: controls the amount of light passing from the slide source Condenser lens: collets and focuses the light from the illuminator onto the slide

11/2/20159 LENSES They magnify the image Objectives: they have 3 or 4 objectives 4X scanner 10X low power 40X high dry 100 X oil emersion lens Ocular lens or eyepiece: it is 10X and it can have a scale

11/2/ ADJUSTMENT KNOBS They are for focusing Coarse adjustment knob: for locating and focusing objects (DO NOT USE for high magnification) Fine adjustment knob: (small) for focusing on higher powers knobs

11/2/ ARM Supports the objective lens

11/2/ CARES OF THE MICROSCOPE When moving the microscope, carry it with 2 hands (one hand to grip the arm and the other under the base Lenses have to be clean with lens paper (to keep them free of oil and dust). Do not use the coarse adjustment when focusing with the higher power objectives

11/2/ PROCEDURES 1- Turn on the illuminator using the on/off switch 2- Turn the nosepiece to bring the 4X objective (scanner) into position 3- Raise the stage into its highest position 4- Place a slide of the letter “e” in the slide clamp on the stage 5- Turn the coarse adjustment knob to bring the “e” into focus 6- Measure the field (the brightly lighted circle that you see when you look through the ocular lens) 7- Center the ”e” in your field of view and then rotate the nosepiece to 10X 8- Use the fine adjustment knob to focus until the image is sharp. Draw the image. Do not use the coarse adjustment 10- Rotate the nosepiece until the 40X. Draw the image

11/2/ SQUAMOUS CELLS Simple squamous peritoneum

11/2/2015ALFONSO A. PINO MD.15 CUBOIDAL CELLS Simple cuboidal kidney

11/2/2015ALFONSO A. PINO MD.16 COLUMNAR CELLS Simple columnar small intestine

11/2/ REMEMBER! GO TO THE TUTORING ROOM AND PRACTICE WITH MODELS