Microscopes & Lab Techniques
What is a light microscope? Light Microscope – a device that uses light to produce an enlarged view of an object An example of a simple microscope is a magnifying glass
What is a compound microscope? Compound Microscope – contains 2 or more objective lenses. – One lens (low power) produces an enlarged image – Another lens (high power) further magnifies the image
Magnification vs. Resolution Magnification – the enlargement of an image Resolution – how sharp or in focus an object is
What is an electron microscope? Electron Microscope – can magnify images more than 250,000 X – Uses electron beams rather than light
A Scanning Electron Microscope
An ant
Leaf from a Black Walnut tree
Pollen
A moth
A human skin cell
A cancer cell
Centrifugation Centrifugation – a process by which materials suspended in a liquid can be separated from each other based on density
Microdissection Microdissection – tiny instruments are used to perform various operations on living cells
Tissue Culture Tissue Culture – the technique of maintaining living cells or tissues in a culture medium outside the body
Chromatography Chromatography – any technique that separates different substances based on their chemical and physical properties
Electrophoresis Electrophoresis – the technique for separating substances made up of particles that have an electrical charge
Spectrophotometry Spectrophotometry – a technique of using light to analyze samples