BY 102 Zach Nolen

 I’m a Master’s student in the Thacker lab  Studying sponge/cyanobacteria relationships  Teaching Portfolio Teaching Portfolio

 Fill out your notecard with the following:  Name (you wish to be called)  Major  Reason taking class (be honest)  1 thing your looking forward to in this class  1 thing your not looking forward to in this class  What’s your favorite food

 Read ahead  Do study guides  Work on lab manual throughout  Keep good notes  Ask questions  Study with others

 Microscopes allow us to view very small objects  There are three main types of microscopes: compound light, dissecting, and electron microscopes.  For this class, we will be working with compound light microscopes.

Pollen Spider Diatoms

 Always carry scope with one hand on the arm and the other under the base.  Always clean scope when you are done.  Always start on the low objective (red), then work your way up in magnification.  Never use the coarse adjustment knob when using an objective higher than red.  If there are any issues with your scope, please let me know immediately.

 Microscopes are very useful tools for biologists.  Not only can they be used to magnify things, they can also be used to measure things.  To calculate the total magnification of your scope, you simply multiply the ocular magnification x objective magnification  Take a few minutes to complete the chart in your lab manual.

 For this exercise, we will be using the letter “e” slides.  You will place the slide on the microscope with the “e” in the correct orientation, then view it through the microscope to observe how the scope transposes images.

 In this exercise, you will be preparing a wet mount of your own cheek cells.  Make sure that you are using a blank slide.  We will be doing a slightly different procedure than listed in the book.  After you have prepared your slide, use your microscope to find a cell on the red objective and let me check it.  After I check it, you will need to use the highest objective (blue) to find the cells for your drawing.  After you have finished your drawing, you can wash your slide off in the sink. You will be using the same slide for other exercises.

 For this exercise, you will be preparing a wet mount of an Elodea leaf to observe plant cells.  Elodea is a freshwater plant that is typically found in ponds.  Follow procedure in the book for slide prep.  You will draw this using the blue objective  After you finish your drawing, you will want to add a few drops of NaCl to the leaf and observe plasmolysis.

 This exercise is demo that I will do to show the process of osmosis.  What is osmosis?  Osmosis is simply the movement of water molecules through a selectively permeable membrane from an area of higher concentration to an area of lower concentration.

 This exercise is just a review of the various parts of plant and animal cells as well as their functions.  Figures 1.8 and 1.9 in your manual have labeled images of both.  You should be able to identify the different parts and also be able to give their function.

1. What are the 3 types of microscopes? 2. Which type will we use in lab? 3. What are 3 differences between animal and plant cells? 4. What is osmosis? 5. What is the purpose of mitochondria in the cell?

 Make sure you properly store your microscope  Clean up your work station  Wipe down your station  Turn in your microscope drawings

 Read Lab exercise 2: Nutrition and the Cell  Do the online prelab  Read the introduction in your manual (viii-xi)  Complete all questions in Lab 1 exercises that we covered, if not already done so