CH. 5 PROKARYOTIC GROWTH
TBL05_01: Some microbial record holders
Figure 05.01: Atacama Desert © Photodisc/age fotostock
Figure 05.02a: Binary fisson of bacteria
Figure 05.02b: A false color TEM of a cell of Bacillus licheniformis undergoing binary fission © Lee Simon/Photo Researchers, Inc.
Figure 05.03: A skyrocketing bacterial population
Growth curve plotted in logs and arithmatically
Figure 05.04: Bacterial growth curve
Figure 05.05a: Bacterial colony on blood agar Courtesy of Dr. J. J. Farmer/CDC
Figure 05.05b: Salmonella typhi culture Courtesy of CDC Note the slimy colonies of Salmonella typhi, denoting capsules
Figure 05.06b: Bacillus anthracis spore © Scott Camazine/Alamy Images
Figure 05.06c: Germinating spore Courtesy of Janice Carr/CDC
Figure 05.07: The formation of a bacterial spore
BACTERIAL GROWTH IS AFFECTED BY MANY FACTORS
TBL05_01: Some microbial record holders
Oxygen can support or hinder growth
Figure 05.09abc: Bacterial cultivation in different gas environments © Scott Coutts/Alamy Images
Figure 05.10: The effect of oxygen on prokaryotic growth
Hyperbaric chamber
Most bacteria like to grow at a neutral pH Optimal pH for growth
TBL05_02: Composition of complex & chemically defined growth media
TBL05_03: A comparison of bacterial media
Figure 05.12a-e: The streak plate isolation method Courtesy of James Gathany/CDC
Population growth can be measured in several ways Turbidity Direct measurements Indirect methods
Counting Bacteria by MPN (most probable number)
Figure 05.13: Direct counting procedure using the Petroff-Hausser counting chamber
Counting bacteria by filtration
Counting bacteria by measuring turbidity in a broth culture
Figure 05.14: The standard plate count © R.A. Longuehaye/Photo Researchers, Inc.
BACTERIAL DEATH Bacteria die at a constant rate
Death Curve