Introduction to the Microscope  Care  Parts  Focusing

How do microscopes help scientists study living things? Microscopes help scientist to observe and study organisms to small to see with the eye alone. They do this by making objects look larger. (magnify)

How are simple microscopes and a compound microscope alike/different? A simple microscope has only 1 lens. It does not magnify things very much. A compound microscope has 2 sets of lenses. It can magnify things times larger than they really are. An electron microscope can magnify objects up to 300,000 times. They do not use lenses, but use electrons to enlarge the image.

Always carry with 2 hands Always carry with 2 hands Only use lens paper for cleaning Only use lens paper for cleaning Do not force knobs Do not force knobs Always store covered Always store covered Keep objects clear of desk and cords Keep objects clear of desk and cords

Place the Slide on the Microscope Place the Slide on the Microscope Use Stage Clips Use Stage Clips Click Nosepiece to the lowest (shortest) setting Click Nosepiece to the lowest (shortest) setting Look into the Eyepiece Look into the Eyepiece Use the Coarse Focus to bring the object in focus. Use the Coarse Focus to bring the object in focus.

How to Use a Microscope 1. Turn the revolving nosepiece to the low power lens. 2. Using the course adjustment knob, lower the body tube to its lowest point. 3. Place the slide on the stage & secure it with the clip. 4. Look through the eyepiece and turn the course adjustment knob backwards slowly so that the lens is moving UP. Object should come into focus.

How to Use a Microscope 5. Take your eye away from the eyepiece & turn the nosepiece to the low power objective lens. 6. Look through the eyepiece & turn the fine adjustment knob slowly. The object should come into sharper focus. 7. Take your eye away from the eyepiece & turn the nosepiece to the high power objective lens.

How to Use a Microscope 8. Look through the eyepiece & turn the fine adjustment knob slowly. The object should come into sharper focus. 9. When observations are complete, turn the nosepiece to the scanner power lens. 10. Remove the slide from the stage. Turn the course adjustment knob to lower the nosepiece to its lowest level.

Medium Power Get in focus on low! Get in focus on low! Do not lower the stage Do not lower the stage Move to medium power and bring into focus while looking through microscope. Move to medium power and bring into focus while looking through microscope. Move to high power. DO NOT LOWER TE STAGE! Move to high power. DO NOT LOWER TE STAGE!

Follow steps to focus using low power and medium power Follow steps to focus using low power and medium power Click the nosepiece to the longest objective Click the nosepiece to the longest objective Do NOT use the Coarse Focusing Knob Do NOT use the Coarse Focusing Knob Use the Fine Focus Knob to bring the slide Use the Fine Focus Knob to bring the slide What can you find on your slide?

What are the parts of a Compound Light Microscope? 1. eyepiece - the lens look through(10X) 2. course adjustment - the large knob 3. fine adjustment - the small knob brings the image into sharpest focus

What are the parts of a Compound Light Microscope? 4. arm - supporting the body tube 5. body tube - holds the eyepiece and the objective lenses. 6. revolving nosepiece - holds the objective lenses and allows them to be turned

What are the parts of a Compound Light Microscope? 7. high power objective lens – Largest lens magnifies the object the greatest amount. (usually 40x) 8. Low power lens - the lens that magnifies the object the least amount ( 4 X) 9. Medium power objective lens – middle lens ( 10x )

What are the parts of a Compound Light Microscope? 10. stage - where the slide is placed 11. clip - holds the slide in place so it doesn’t move 12. diaphram - controls the amount of light entering the field of view

What are the parts of a Compound Light Microscope? 13. light source - sends light through the object being viewed. 14. base - the bottom supports the rest of the microscope

Making a Wet Mount Slide 1. Use dropper to place a drop of water on the center of a clean slide. 2. Use tweezers to lay specimen on the drop of water. 3. Gently touch the cover slip to the edge of the drop of water to cover the specimen & the water.

Appearance of the Specimen Objects appear upside-down & backward Objects appear upside-down & backward Movement appears to be in opposite direction than actual movement 1 2 Movement appears to be in opposite direction than actual movement 1 2 move slide move slide slide appears to move slide appears to move 2 1

Field of View Field of view is the area (circle) that you see when looking through the eyepiece Field of view is the area (circle) that you see when looking through the eyepiece The big black pointy thing is a POINTER!!

Calculating Magnification 1. Find the power of the lens. It is found on the side of the lens. Magnification power of a lens is always identified by the label of x (10x, 1000x) 2. Multiply the power of the eyepiece by the power of the objective lens. 3. Examples: eyepiece obj. lens eyepiece obj. lens 10x times 100x 10x times 100x 10x times 50x 10x times 50x 10x times 40x 10x times 40x