Precursor m/z 740.44 double charge iTRAQ ratio 114:113 1.78 iTRAQ ratio 116:115 1.40 iTRAQ ratio 121:119 2.83 Precursor m/z 1831.95 triple charge iTRAQ.

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Precursor m/z double charge iTRAQ ratio 114: iTRAQ ratio 116: iTRAQ ratio 121: Precursor m/z triple charge iTRAQ ratio 114: iTRAQ ratio 116: iTRAQ ratio 121: A B Supplemental Figure S1

Precursor m/z triple charge A B Precursor m/z triple charge Supplemental Figure S2

Precursor m/z triple charge A B Precursor m/z charge Supplemental Figure S3

Precursor m/z triple charge A B Precursor m/z triple charge Supplemental Figure S4

Precursor m/z double charge A B Precursor m/z double charge Supplemental Figure S5

Precursor m/z charge Precursor m/z triple charge A B C Precursor m/z triple charge Supplemental Figure S6

Supplemental Figure S1 - Protein identification and relative quantification of dihydrodipicolinate reductase (Swiss-Prot:B8C5E0) (A) The fragmentation spectrum of MEIVSSLAGLR, a peptide fragment identified with high confidence (>95 %) is shown with the reporter ion signals used for the BaP-exposed T. pseudonana (iTRAQ 114, 116 and 121) when compared to control conditions (iTRAQ 113, 115 and 119). (B) Fragmentation spectrum and reporter ion signals are also shown for TVYAEGTADAVK, a peptide fragment with lower confidence. Supplemental Figure S2 - Protein identification and relative quantification of a predicted NonF-related protein; DJ-1/PfpI family (Swiss-Prot:B5YN08) (A) The fragmentation spectrum of DSEELAVQLEK, a peptide fragment identified with high confidence (>95 %) is shown with the reporter ion signals used for the BaP-exposed T. pseudonana (iTRAQ 114, 116 and 121) when compared to control conditions (iTRAQ 113, 115 and 119). (B) Fragmentation spectrum and reporter ion signals are also shown for LKELGGK, a peptide fragment with lower confidence. Supplemental Figure S3 - Protein identification and relative quantification of inorganic pyrophosphatase (Swiss-Prot: B8C6T9) (A) The fragmentation spectrum of VIAIATDDELAK, a peptide fragment identified with high confidence (>95 %) is shown with the reporter ion signals used for the BaP-exposed T. pseudonana (iTRAQ 114, 116 and 121) when compared to control conditions (iTRAQ 113, 115 and 119). (B) Fragmentation spectrum and reporter ion signals are also shown for MAGEVIGETHEAWK, a peptide fragment with lower confidence. Supplemental Figure S4 - Protein identification and relative quantification of porin/voltage-dependent anion-selective channel (Swiss-Prot:B8BQH4) (A) The fragmentation spectrum of AGPVGVTVETER, a peptide fragment identified with high confidence (>95 %) is shown with the reporter ion signals used for the BaP-exposed T. pseudonana (iTRAQ 114, 116 and 121) when compared to control conditions (iTRAQ 113, 115 and 119). (B) Fragmentation spectrum and reporter ion signals are also shown for FSYAGLNFDK, a peptide fragment with lower confidence. Supplemental Figure S5 - Protein identification and relative quantification of silicon transporter 1 (Swiss-Prot:Q0QVM8) (A) The fragmentation spectrum of DALAEDA, a peptide fragment identified with high confidence (>95 %) is shown with the reporter ion signals used for the BaP-exposed T. pseudonana (iTRAQ 114, 116 and 121) when compared to control conditions (iTRAQ 113, 115 and 119). (B) Fragmentation spectrum and reporter ion signals are also shown for LTPFNILR, a peptide fragment with lower confidence. Supplemental Figure S6 - Protein identification and relative quantification of rubisco expression protein (Swiss-Prot:B8LEU2) (A) The fragmentation spectrum of LLNLLDEELVGLAPVK, a peptide fragment identified with high confidence (>95 %) is shown with the reporter ion signals used for the BaP-exposed T. pseudonana (iTRAQ 114, 116 and 121) when compared to control conditions (iTRAQ 113, 115 and 119). Fragmentation spectra and reporter ion signals are also shown for the peptide fragment with lower confidence with sequences IANHIDFPDYTVEELLIIAK (B) and EIAALLLIDK (C).