M.Jabari 2005

Pipette Graduated (Measuring ) To deliver (EX) To contain (IN) (Wash with solution ) I Line II Line Volumetric

% CV must be less than 0.3 % % Bias must be less than 0.6% Ideal % Bias 0 – 0.6% is acceptable

Expected value Observed value _ % Bias = X 100  (x- M ) 2 n - 1 SD = % CV = X 100 Mean SD

GRAVIMETRY METHOD COLORIMETRY METHOD

 First weight a dish.  Then with a pipette that you want caliber it transfer DW into the dish and weight that.  Repeat 10 time this action and record weight.  Caculate each time pure weight of DW that transfer with pipette.  Caculate SD, %CV and % Biass.

(X – mean) 2 Mean =  (x- M ) 2 n - 1 SD = % CV = X 100 Mean SD  (x- M ) 2 = = 0.16= 0.4 = 0.04 Acceptable 0.04 < 0. 3

Mean of Expected weight Mean of Observed weight _ % Bias = X (X – mean) % Bias = X 100 _ = _ Acceptable < 0. 6

Methyl orange 492 nm Phenol red 405 nm

 For 1 ml pipette :  In a ballon class A.  Prepare 0.1 gr /100 ml (stock ).  Dilute 1 : 11 and measure absorbance.  Select 10 tube and add 10 ml DW in each tube. (for 2 ml add 20 ml DW )  Add 1 ml stock reagent into each tube.  Measur absorbance and record it.

Mean of Expected OD Mean of Observed OD _ % Bias = X % Bias = X 100 _ = 1.58 _ (X – mean) Absorbance of 1:11 in class A ballone is > 0.6 No acceptable

Mean = 435  (x- M ) 2 n - 1 SD = % CV = X 100 Mean SD  (x- M ) 2 = 358 = 39= 6.2 = (X – mean) > 0.3 No acceptable

GRAVIMETRY METHOD COLORIMETRY METHOD

 Each sampler shoud assese 3 – 4 time annually.  Acceptable % CV up to 3%  Acceptable % Biass up to 5%

 First weight a dish.  Then with a sampler that you want caliber it transfer DW into the dish and weight that.  Repeat 10 time this action and record weight.  Caculate each time pure weight of DW that transfer with sampler.  Caculate SD, %CV and % Biass.

(X – mean) 2 Mean =  (x- M ) 2 n - 1 SD = % CV = X 100 Mean SD  (x- M ) 2 = 16.1 = 1.78= 1.33 = < 3 Acceptable

Mean of Expected weight Mean of Observed weight % Bias = X % Bias = X 100 _ = 0.7 _ (X – mean) < 5 Acceptable

Methyl orange 492 nm Phenol red 405 nm

llll  For ml sampler :  In a ballon class A.  Prepare 0.1 gr /100 ml (stock ).  Dilute 1 : 101 and measure absorbance.  Select 10 tube and add 1 ml DW in each tube. ( for 20  l,50  l,100  l add 2, 5, 10 ml DW )  Add 10  l stock reagent into each tube.  Measur absorbance and record it.

Mean of Expected OD Mean of Expected weight Mean of Observed OD _ % Bias = X % Bias = X 100 _ = 0.74 _ (X – mean) Absorbance of 1:101 in class A ballone is < 5 Acceptable

Mean =  (x- M ) 2 n - 1 SD = % CV = X 100 Mean SD  (x- M ) 2 = 36.4 = 4.04= 2.01 = (X – mean) < 3 Acceptable

llll  For more than 100 ml sampler :  In a ballon class A.  Dilute 1 : 11 from stock reagent.