New method to differentiate human peripheral blood monocytes into insulin producing cells : Human hematosphere culture Supplementary Figures.

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New method to differentiate human peripheral blood monocytes into insulin producing cells : Human hematosphere culture Supplementary Figures

Supplementary Fig. 1. (Kim) PBMC isolation & Generation of Hematosphere Peripheral blood from healthy volunteer Ficoll-Hypaque Isolation of MNCs using Ficoll 3D culture High cell density Ultra low attach dish Mononuclear cells(MNCs) layer Culture of Blood derived Hematosphere EBM-2 (5%FBS) Supplementary Fig 1. Generation of BBHS (A) Schematic protocol for generation of hematosphere in human.

Unstained BBHSBBHSStage-1 Stage-2Stage-3Stage-4 Supplementary Fig. 2. (Kim) Supplementary Fig 2. Detection of insulin protein via DTZ staining The intensity of insulin staining via DTZ (binding of zinc ion to insulin protein) increased gradually throughout the distinct four-stage of IPCs-development. Scale bar represents 10 ㎛