Ann Bucklin University of Connecticut – Avery Point, USA CMarZ Symposium and Steering Group meeting IOCAS, Qingdao CHINA – May 11-13, 2010.

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Presentation transcript:

Ann Bucklin University of Connecticut – Avery Point, USA CMarZ Symposium and Steering Group meeting IOCAS, Qingdao CHINA – May 11-13, 2010

CMarZ Steering Group includes 23 people from 14 countries Shown here with Japanese colleagues at the University of Tokyo, Japan November, 2008 CMarZ Steering Group

  Integrated morphological and molecular systematic analysis: CMarZ cruises have included expert taxonomists and barcoders, who work together toward accurate descriptions of zooplankton species diversity, including discovery and description of new species.   Global surveys: CMarZ Steering Group members have worked to achieve global sampling from ships of opportunity and dedicated cruises.   Biodiversity hotspots: CMarZ has focused on biodiversity hotspots (areas of high probability for species discovery) in the deep sea, coastal regions of Southeast Asia, and other under-sampled ocean regions. CMarZ Goals

Zooplankton Diversity

Analysis of Global Biodiversity Since 2004, CMarZ has completed more than 90 cruises and collected samples from > 12,000 stations; 6,500 archived samples have been available for analysis.

CMarZ Biodiversity Surveys R. Hopcroft M. Kuriyama & H. Matsuura Taxonomic Analysis DNA Barcoding at Sea ABI 3130 Capillary Sequencer PCR machines U. Piatkowski & H. Ossenbrügge (left); P. Batta Lona (below) B. Ortman & P. Batta Lona CMarz has carried out comprehensive biodiversity surveys, with at-sea taxonomic analysis of samples, photography of living specimens, and DNA barcoding.

CMarZ Zooplankton Sampling

Limacina helicina Hippopodius hippopusSalpa cylindrica DNA Barcoding of Zooplankton Gold standard barcoding (determination of ~548 base-pair sequence of mtCOI from identified specimens) will yield a ROSETTA STONE for identification of zooplankton species. Sargasso Sea barcodes: Bucklin, Ortman, et al. Rosetta Stone for zooplankton (2010) Deep-Sea Research II Environmental barcoding of zooplankton (sequencing of COI from bulk environmental samples) allows rapid biodiversity assessment; species are identified using barcode library. Barcodes for sample from Pohnpei Island, Micronesia, Equatorial Pacific Ocean: Machida Hashiguchi, et al. (2009) BMC Genomics

  CMarZ new species descriptions (published):   Estimated new species ‘discovered’ = over 100.   Group-by-group analyses include: - Handbook of the Radiolaria. Monographic publication now in press. [Demetrio Boltovskoy] - Taxonomy, phylogeography, and phylogeny of chaetognaths. [Annelies Pierrot-Bults] - Atlantic atlas for the planktonic ostracods: Publ. on NHM (London, UK) website. [Martin Angel] New Species Discovery Russ Hopcroft & Dhugal Lyndsay Janet Bradford Grieve Martin Angel D. Boltovskoy

Ballast Water in Argentine Ports : Transoceanic ships’ ballast water was monitored; salinity is measured; plankton samples are collected and distributed among 10 specialists in various taxonomic groups for identification of freshwater and marine zooplankton. [Demetrio Boltovskoy - Argentina] Impacts on Society Impacts of a Marine Bioinvasion : Sampling from the Caspian Sea showed striking impacts of the invasive predatory ctenophore Mnemiopsis leidyi. Satellite- derived chlorophyll-a concentration was significantly correlated with ctenophore biomass. By consuming grazing zooplankton, M. leidyi may have caused levels of Chl a to rise to record high values (9mg /m 3 ). [Ahmet Kideys - Turkey] Kideys, A.E. et al. (2008) Research Letters in Ecology

CMarZ Education & Outreach JSPS-CMarZ Training Course, Univ. Philippines, Los Baños, Dec Rosamma Stephen at AWI, July 2006 CMarZ has organized >358 E&O events attended by >1,400 people, excluding television audiences. Activities have included: Advanced training in taxonomy and DNA barcoding for researchers, Field trips for elementary school students, Newspaper and magazine articles for the general reader. Asahi Press, Jan. 2010

Visualization & Communication

Goals of this Meeting 1. 1.CMarZ Symposium - New results and findings - New perspectives and analysis 2. 2.CMarZ Synthesis - Sample collection and analysis - Data collection and analysis - Reports and publications - Education and outreach - Communications 3. 3.CMarZ Synthesis Products and Plans 4. 4.Planning for CoML finale and CMarZ wrap-up - Posters and presentations - Proposals for new funding and next steps.