Prof. Jyotsna Agarwal Dept Microbiology KGMU

Slides:



Advertisements
Similar presentations
Management of Streptococcal Pharyngitis: Role of the Laboratory and POC Testing Arthur E. Crist, Jr., Ph.D. Director, Clinical Microbiology York Hospital.
Advertisements

Streptococcal Diseases
Upper respiratory tract infection: Streptococcus pyogenes. Neisseriae meningitidis. Haemophilus influenzae, and H parainfluenzae. Bordetella pertussis.
Scarlet fever Introduction 1 A kind of acute infectious 1 A kind of acute infectious disease of respiratory tract disease of respiratory tract 2 Group.
1 Streptococci (Gram positive cocci) Lecture 37 Streptococci (Gram positive cocci) Lecture 37 Faculty: Dr. Alvin Fox.
Hugh B. Fackrell Filename:Strept.ppt
Bipolar Gram Negative Rods
Cocci of Medical Importance
Streptococci Characters of Streptococci Gram positive cocci
Micrococci Marphology: _ Gram +ve cocci Arrangement : Tetrades _ _ _ _ _ Non motile, non capsulated, non sporulated Habitat : May be normal present in.
Streptococcaceae.
Biology 431 Gram (+) Cocci Chapters Staphylococcus Major groups - coagulase (+) aureus vs. (-) others. External Structures Capsule - polysaccharide,
Streptococci Eva L. Dizon, M.D.,D.P.P.S Department of Microbiology.
Isolation and Identification of Gram Positive Cocci
Medical bacteriology:
Streptococcus and enterococcus (greoup D Strept)
Streptococcus Species
MICROBIOLOGY DIAGNOSTIC OF MICROORGANISMS RELATED TO CARDIAC INFECTIONS Microbiology Department.
Unit 4 Part 3 Streptococcal Serology
Streptococci Lecture 5 Medical Microbiology SBM 2044.
GRAM POSITIVE COCCI erly Gram positive and negative bacteria: The cell wall very different:. Peptidoglycan  very strong, thick and rigid.. Teichoic acid.
Streptococcus Basmah almaarik Lab # 6.
Streptococcus.  Low G+C  Cocci pairs/chains  FA  Non motile, NSF  Capnophiles  Catalase (-)  Peroxidase  Hemolysins  Lancefield Groups 18 antigens.
Gram Positive Bacteria and Clinical Case Studies II
Diagnosis of streptococci Compiled by Thamer Hamdan Compiled by Thamer Hamdan M.Sc. Clinical Microbiology and Immunology M.Sc. Clinical Microbiology and.
Streptococci.
Streptococcus Gram+ cocci In chains.
Streptococci. Introduction Pyogenic pathogens - nonmotile, catalase negative, Gram positive cocci in chains.
Dr. Tamer Bedair Lecturer of Medical Microbiology and Immunology.
Streptococcus Tao Chuanmin
PHARMACEUTICAL MICROBIOLOGY -1I PHT 313
E. coli Prof. Jyotsna Agarwal Dept. Microbiology KGMU.
Practical no.2 - winter term- Streptococcal infections Diagnostical model - tonsilitis, febris reumatica, streptococcal pneumonia Microscopy of bouillon.
Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae and Streptococcus pyogenes on mannitol salt agar plates (containing.
Streptococci Lecture 4 Medical Microbiology SBM 2044.
Chapter 23 – Streptococcus. Introduction Gram + cocci in chains Most are facultative anaerobes –Some only grow with high CO 2 Ferment carbs. to lactic.
Nora Al-Kubaisi Gram’s +ve Cocci Irregular Clusters Tetrads Chains or Pairs Staphylococci Micrococci Streptococci.
Streptococcaceae I Jeanne Filbey MT(ASCP)
Staph/Strep Peter Coschigano, Ph.D. Department of Biomedical Sciences OUCOM.
Prof. Dr. Asem Shehabi Faculty of Medicine University of Jordan
LAB 7: RESPIRATORY SYSTEM. RESPIRATORY SYSTEM: UPPER AND LOWER.
Bacterial Respiratory Infection (3rd Year Medicine)
Clinical Microbiology ( MLCM- 201) Prof. Dr. Ebtisam.F. El Ghazzawi. Medical Research Institute (MRI) Alexandria University.
General Microbiology Laboratory Isolation and Identification of Gram Positive Cocci.
COLLECTION OF SAMPLES FOR BACTERIOLOGICAL EXAMINATION
Staphylococci DON XAVIER N.D.  Cocci.  Gram positive.  Non motile.  On NA they form pigmented colonies.  Haemolytic.  Enzyme production.  Ferment.
S TREPTOCOCCUS AND E NTEROCOCCUS. They member of family streptococcacea. Catalase and cytochrome enzyme (oxidase) differentiated them from other micrococal.
Streptococcus IMPORTANT PROPERTIES 1-streptococci are spherical gram-positive cocci. 2-arranged in chain or pairs. 3-all streptococci are catalase negative.
Streptococcus agalactiae (GBS)
Lec.3 Genus: Streptococcus Streptococci are Gram positive spherical cocci. Classification of Streptococci The classification of Streptococci has been based.
Bacterial Identification
5  Arrangement of cocci in pair or long chains  Non-spore forming, non-motile  Capsule, slime layers  Facultative anaerobes  Catalase(-), peroxidase(+)
Medically Important Bacteria Gram Positive Cocci.
Streptococcaceae family Classification - Includes 7 genera: –Streptococcus – a major cause of human infections –Enterococcus – frequent cause of human.
Streptococci & Corynebacteria
The Staphylococci: Isolation and Identification
Streptococcus and Enterococcus
STREPTOCOCCI By Eric S. Donkor.
Gram-Positive Cocci Part 6-A
Streptococcal Serology
Streptococcus(gram positive coccus) Dr. Hala Al Daghistani
GRAM POSITIVE COCCI Gram positive and negative bacteria:
Streptococcus pyogenes
Introduction to Microbiology
II- Streptococci Characters of Streptococci Gram positive cocci
STREPTOCOCCUS BY MBBSPPT.COM
Streptococcus species
assist. Prof. Zainab Abdul jabar Aldhaher
Presentation transcript:

Prof. Jyotsna Agarwal Dept Microbiology KGMU Streptococcus Prof. Jyotsna Agarwal Dept Microbiology KGMU

Streptococci Characters of Streptococci Gram positive cocci Size 1µm Chains or pairs Non motile Non spore forming Facultative anaerobes Fastidious Catalase negative

Classification of Streptococci According to: Oxygen requirement Anaerobic (Peptostreptococcus) Aerobic or facultative anaerobic (Streptococcus) Hemolysis on Blood Agar- α, β, Ƴ Lancefield classification- for β hemolytic streptococci, based on C antigen

Hemolysis on Blood Agar Hemolysis on BA -hemolysis Partial hemolysis Green discoloration around colonies e.g. S. pneumoniae & S. viridans -hemolysis Complete hemolysis Clear zone of hemolysis around colonies e.g. Lancefield Group A & B (S. pyogenes & S. agalactiae) -hemolysis No hemolysis e.g. Group D (Enterococcus sp.)

Hemolysis on Blood agar

Lancefield Classification- β hemolytic Classification based on C- carbohydrate antigen of cell wall 20 Lancefield groups from A-H & K-V One or more species per group Group A- Further subdivided based on M, T, R protein (Griffith typing) in to 80 sertypes

Disease caused by S. pyogenes (Group A) Suppurative Non-Invasive Pharyngitis - inflammation of the pharynx, tonsillitis, otitis media, mastoiditis, rarely meningitis Pyoderma- Impetigo- localised pus-producing lesions usually occur on face, arms, or legs

Suppurative Invasive Erysipelas- diffuse infection of skin, involves superficial lymphatics. well demarcated borders Cellulitis- infection of skin & subcutaneous tissue, spreading

Toxin Mediated Diseases Scarlet fever- rash begins on chest & spreads across body Necrotizing fasciitis-Pyrogenic exotoxin produced by some trains of S. pyogenes- Can lead to DIC. Streptococcal Toxic Shock Syndrome

Non Suppurative complications or sequelae Rheumatic fever: following pharyngitis carditis, poly arthritis, damage to heart valves potentially fatal Acute Glomerulonephritits- following skin infection Immune complex mediated disease inflammation of glomeruli due to Ag-Ab complex deposit on basement membrane Clinically- Hematuria, Proteinuria, Hypertension Good prognosis

Pathogenesis & Virulence Factors Structural components M protein inhibits phagocytosis of bacteria Lipo teichoic acid helps in adhesion of bactera Capsule- camouflages bacteria Enzymes Streptokinases- fibrinolysin Deoxyribonucleases Hyaluronidase Pyrogenic / Erythrogenic exotoxin Hemolysins Streptolysin O- Oxygen labile, antigenic Streptolysin S- serum soluble Facilitate spread of streptococci in tissues

Lab Diagnosis Sample -Throat swab, pus / swab Gram Stain- GPC in chains Culture on Blood agar- beta hemolytic colonies, catalase negative

Bacitracin sensitivity Principle: for presumptive identification of group A distinguish between S. pyogenes from other beta hemolytic streptococci Strep. Pyogenes sensitive to Bacitracin giving zone of inhibition around disk

Differentiation of -hemolytic streptococci Lanciefield Classification Bacitracin susceptibility Test Specific for S. pyogenes (Group A) CAMP test Specific for S. agalactiae (Group B)- normal flora of genital tract in women, can cause neonatal meningitis

CAMP test Principle: Group B streptococci produce extracellular protein (CAMP factor) CAMP act synergistically with staphylococcal -lysin to increase lysis of RBCs

Summary Streptococcus general characters Lancefield Classification Toxins / Enzymes of S. pyogenes Infections / Sequelae caused Laboratory Diagnosis