Chapter 12 Processing pre-mRNA and Nuclear Transport

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Presentation transcript:

Chapter 12 Processing pre-mRNA and Nuclear Transport By: Rachael Hattrup

Processing of eukaryotic Pre-mRNA 1) 5’ capping with 7-methylguanosine 3’cleavage at Poly (A) site Polyadenylation added 250 in mammals 150 insects 100 yeasts 4) RNA splicing Introns removed and exons kept.

5’ Capping

Overview of mRNA processing in Eukaryotes

Macromolecular Transport into Nucleus Nuclear envelope Nuclear Pores Nuclear pore complex (NPC) Nucleoporins

Nuclear Pore Complex

Macromolecular Transport into Nucleus 2 Importins bind to create nuclear-import receptor -Importin binds to Nuclear-localization signal (NLS) of “cargo” protein 2) -importin interacts with FG nuclear proteins to diffuse through NPC

Macromolecular Transport into Nucleus cont… Ran-GTP interacts with importins causing conformational change creating less affinity for NLS Ran-GTP transports importin out to cytoplasm

Transport into Nucleus

Macromolecular Transport out of Nucleus 1) Protein exportin 1 binds to Nuclear-export signal (NES) bound to “cargo” protein and Ran-GTP 2) Difusses through NPC Ran-GTP converted to Ran-GDP releasing the complex

Transport out of Nucleus

The End Questions?????

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