H.Özge Kızılsu 1 SSME-518 Instructional Technology and Material Development Assignment2 Presented by: Harika Özge Kızılsu Presented by: Harika Özge Kızılsu

H.Özge Kızılsu 2 Outline Introduction to enzymes How enzyme work  Activation energy Factors effecting enzyme activity  Temperature  pH  Enzyme concentration  Substrate concentration

H.Özge Kızılsu 3 Introduction to enzymes Enzymes are usually proteins Enzymes are catalysts In general, names end with suffix “ase”

H.Özge Kızılsu 4 Introduction to enzymes Catalyst Time for reaction Yes1 second No3 x years 3 x years is 500 times the age of the earth!!!!

H.Özge Kızılsu 5 Introduction to enzymes Enzymes: increase the rate of a reaction not consumed by the reaction act repeatedly to increase the rate of reactions are often very “specific” for the substrate Reactants also called “substrates” of enzyme

H.Özge Kızılsu 6 How enzyme work Enzymes physically interact with their substrates Substrates bind to the enzyme’s active site Enzyme reaction path:

H.Özge Kızılsu 7 How enzyme work Lock and Key Model Substrate fits into an active site perfectly. (key) (lock) (key) (lock) click to see animation of model

H.Özge Kızılsu 8 Activation energy is: the energy required to start a reaction. How enzyme work

H.Özge Kızılsu 9 Factors effecting enzyme activity  Temperature  pH  Enzyme concentration  Substrate concentration

H.Özge Kızılsu 10 Temperature C (optimum temperature)

H.Özge Kızılsu 11 pH

H.Özge Kızılsu 12 Enzyme concentration Substrate concentration constant

H.Özge Kızılsu 13 Substrate concentration click to learn more Enzyme concentration constant

H.Özge Kızılsu 14 Thank YOU Thank YOU

H.Özge Kızılsu 15 References enz/enzymology.htmhttp://fig.cox.miami.edu/~cmallery/255/255 enz/enzymology.htm ns/buildings/doors/6038e/ ns/buildings/doors/6038e/