rh Lecture 3 Slides

Michaelis-Menten Equation V o = V max S K m + S again, a rectangular hyperbola Maud Menten rh

Michaelis-Menten Equation V o = V max S K m + S rh when there is no S, V 0 is also 0 as S gets big, V 0 approaches V max saturable

fig 6-11

box 6-1 Lineweaver-Burk: 1/S vs. 1/V hyperbola becomes a line

1/V o = V max S K m + S rhM & M inverted Lineweaver-Burk Plot

box 6-1 Lineweaver-Burk: 1/S vs. 1/V hyperbola becomes a line

In class I show a lot of fancy uses of the LB plot, but you just need to know the basic double reciprocal plot!!!

box 6-1 Lineweaver-Burk: 1/S vs. 1/V hyperbola becomes a line

BIOENERGETICS or Free energy and life

part II intro-1

part II intro-3

part III intro-4

patterns in metabolism there are many…

THE STORAGE and USE of CHEMICAL ENERGY in CELLULAR METABOLISM

 G =  H - T  S Gibbs free energy enthalpy (heat) entropy (order) “delta gee”- The change in free energy

entropy from increased numbers

? - 1 atm pressure - water at 55M - pH = 7 -from initial concentrations of 1M - temperature 25 o C

K’ eq is predicted by  G’ o … table 13-3

table 13-2 A 10-fold change in Keq for each 1.4 kcal/mole

Actual free energy for a reaction concentration ratio when substrates are high- negative when products are high- positive

Actual free energy for a reaction concentration ratio Henri-Louis Le Chatlier

adenosine triphosphate… ATP

The 20$ bill of the cell!!!

table 13-4

Hydrolysis of ATP fig 13-11

table 13-6

fig 13-9

[ATP] [ADP][P i ] Actual ATP hydrolysis energy standard free energy= kJ/mole actual free energy= kJ/mole in erythrocyte

pg 494 free energies are additive!

spontaneity determined by whole set of reactions fig 13-11

What is needed to lift this 3 kg dog into the air? Something heavier than 3 kg!!!

free energies are additive! 11kg 68kg riddle: how do you lift 11 kg? ans: lower a weight > 11kg! In metabolism, it is all about the coupling mechanism

spontaneity determined by whole set of reactions (fig ed 4)

a mechanism of energy coupling fig 13-8

Much of metabolism is about LEAVING GROUPS!!! fig 13-8 C.R.H. Raetz

fig 13-10

adding a good leaving group to form a sigma bond… palmitoyl-CoA + H 2 Opalmitate + CoA-SH This reaction is spontaneous in this direction … so how do we make run the reaction backwards? Ans: couple it to the hydrolysis of ATP

making a good leaving group to form a sigma bond…

driving fancy chemistry by… making a good leaving group! box 13-2