“BSP, May I Be Excused? My Brain Is Full.” Gary Larson – The Far Side ASSESSMENT

UCLA Instructional Laboratories: Where Leading-Edge Science Education Is Always Bruin Jordan Moberg Parker Research Immersion Lab in Virology

Virus Discovery – A Platform for Ultra-Structural Investigations & Genome Annotation. Students will discover a unique bacterial virus (phage) and characterize viral particles by electron microscopy (EM). Students will prepare viral DNA for 454 DNA sequencing of viral genomes. Students will annotate the genome of a novel bacterial virus. RESEARCH PROJECT:

Learning Objectives 1.Demonstrate knowledge of key disciplinary concepts & their relationship to biological systems. 2.Demonstrate ability to Critically read scientific literature

Objective: 1.Demonstrate knowledge of key disciplinary concepts & their relationship to biological systems. Measurable Outcomes: Describe key steps of the lysogenic lifestyle. Explain why lysogenic phage plaques often demonstrate turbid or “bull’s eye” morphologies while lytic phage plaques are clear. Recognize role of lysis and lysogeny in shaping environmental systems Learning Objectives and Outcomes

Objective: 2.Demonstrate ability to Critically read scientific literature Measurable Outcomes: Summarize the purpose or goal of a study. i.e. Identify gaps in the knowledge. Identify the specific hypothesis that is tested in each experiment or the scientific question asked. Illustrate key experimental procedures and connect to expected results. Design a follow-up experiment for the study. Learning Objectives and Outcomes

CREATE Approach to Primary Literature Sally G. Hoskins, City College of the City University of New York

in-class activities out-of-class activities T1 – Warm Up Q: Plaque formation T2 – Lytic Cycle: Lecture/Discuss/Video T3 – 1 min Paper on Lysogeny video T4 – Concept Map lysogeny T5 – 1 min Paper Wrap UP R1 – Warm up Q: Phage/biosphere R2 – Discuss Muddiest points with Team R3 – Mini-lecture: Methods; JITT R4 – Elucidate hypotheses and Analyze data in Teams using Analysis Template (Hoskins) R5 – Whole class discussion Table 1/Figure 1 Williamson 2008 W1 – Revise Concept map to include paper intro ideas W2 – Submit 3 Muddiest Points W3 – Draw artoon Expt. cartoons and Annotate Figures F1 – Revise Concept Map to include Williamson figures as nodes F2 – Think of next experiments 1.Follow-up to this study 2.Team phage expt. feasible to execute M1 – Phage Lifecycle Lit Review Reading Assignment M2 – Content Check (short answer/online) LALA LALA FAFA FAFA SASA SASA LALA LALA FAFA FAFA LALA LALA SASA SASA LALA LALA FAFA FAFA LALA LALA FAFA FAFA SASA SASA FAFA FAFA SASA SASA Formative Assessment Summative Assessment Learning Activity FAFA FAFA LALA LALA FAFA FAFA FAFA FAFA FAFA FAFA LALA LALA LALA LALA LALA LALA FAFA FAFA LALA LALA FAFA FAFA LALA LALA FAFA FAFA LALA LALA C R E A T E OBJECTIVE 1 Lysogeny OBJECTIVE 2 - CREATE

Echols (1986) 1. Briefly outline the general features of lambda development. 2. What the three functions of the lamba cII protein, and what is the effect of cII concentration on the choice of the lytic or lysogenic pathway? 3. Briefly, what is the mechanism of mytomycin C and UV induction of prophage? You may have to look at outside resources to fully answer this question. Phage Lifecycle Content Check M2

Discussion Questions A plaque forms on a lawn of bacteria when I.bacteriophages adsorb to the host cells. II.phages replicate their genome, produce capsid proteins, and assemble progeny virions. III.phage lyse the host cells, releasing progeny virions. What factors may influence the appearance of plaques? T1

The expected relationships between plaque size and various phage traits Gallet et al. BMC Microbiology :181 T1

Plaque Formation – GFP expressing Virus Science 12 February 2010: Vol. 327 no pp T2

Discussion Question Why does the expression of Green Fluorescent Protein (GFP) lag behind the infection front (CPE – cytopathic effects)? T2

Bacteriophage lambda lysis and lysogeny Proc Natl Acad Sci U S A Dec 30;105(52): T3

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