FSCCD40 mediumFluCL097 25 %82 %97 % SSC FSC GEN2.2 cells B mediumFluCL097CpG ACpG B 0 20 40 60 80 100 GEN2.2 GENshTLR7 % CD40 positive cells TLR7TLR9 0.0.

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FSCCD40 mediumFluCL %82 %97 % SSC FSC GEN2.2 cells B mediumFluCL097CpG ACpG B GEN2.2 GENshTLR7 % CD40 positive cells TLR7TLR GEN2.2 GENshTLR7 relative Ct A CD Figure 1 95 %85 % FluCL097pDC CD40 medium CD123 FSC 20 % BDCA2

A C B mediumFluCL IFN α IL-6 IL-8 TNFα pg/ml medium Flu IFN  0.2 %56 % CL097 7 % BDCA-4 Figure 2 DE mediumFluCL097

Figure 3 AB CD 0.5h2h4h24h medium Flu CL097 IFN α (pg/ml) EF

Figure 4 AB mediumFluCL097 IFN α well transwell % TRAIL positive cells C mediumFluCL097 IFN α well well + anti-IFNα/β transwell transwell + anti-IFNα/β % TRAIL positive cells D

Figure 5 A B C medium FluCL097IFN  p-STAT1 p-STAT2 actin 0.5h 2h 0.5h 2h 0.5h 2h 0.5h 2h phospho-STAT1 FSC medium Flu CL097 IFN  5 % 30 % 29 % 65 % D medium FluCL097 IFN 

Figure 6 AB CD medium FluCL097 IFN  E mediumFluCL097 IFN  medium LY SB CXCL10 (pg/ml) F medium Flu SSC CL097 2 % TRAIL IFN  4 % 1 %3 %85 % 94 % 92 % medium SB LY 7 %10 % 90 %