How to use Image J software in quantitative analysis of western blots.

Slides:

Advertisements

Similar presentations

Introduction to GIMP. Overview Opening GIMP Default GIMP Layout Layers Duplicating Layers Adding Color to Layers Layer Order Opacity Selection Tools Selection.

Advertisements

Working with Profiles in IX1D v 3 – A Tutorial © 2006 Interpex Limited All rights reserved Version 1.0.

How to Create a Transparent Image. Locate an image Locate an image, right-click on it and select “Save image as...” or “Save picture as…”

Comic Strips in MS Word Or Powerpoint.

EXCEL Spreadsheet Basics

Boardmaker 6 Basic Tutorial

Bitmap Editing – Lesson 1

Drawing Objects with Illustrator 1.Start a new image in RGB mode. 2.Size 1024 X Unit = pixels 4.Go to View > Show Grid to turn on the grid. 5.Go.

Using MS Paint by A. Satariano Launching MS Paint Click on the Start Button Move the mouse up to the Programs Folder. Then move the mouse up to the Accessories.

Creating a centre square design in Photoshop 1.Skills and techniques overviewSkills and techniques overview 2.Steps 1–8: the first segmentSteps 1–8: the.

Lab 08 Introduction to Spreadsheets MS Excel

Using Powerpoint to Create Interface Prototype Copy & Paste Interface Designs –Use Screen Capture to Copy Existing Interface –“Print Screen / SysRq” Button:

Mr. Potato Head The objective of this tutorial is to cut an image and assemble Mr. Potato Head using various tools in Adobe Photoshop Elements 2.0.

Copyright © Texas Education Agency, All rights reserved.1 Digital and Interactive Media Photo Editing Basics.

Creating Tessellations With Paint. Open Microsoft Paint. Go to: Start, Programs, Accessories, Paint.

Lesson 3: Removing objects. Starter: Open the powerpoint from O:\ Drive 1. Double click “My Computer” on the desktop 2. Double click “O:\” 3. Double click.

Inverted Colors Photo Effect Before: After:. Step 1: Select Any People In The Image And Place Them On A Separate Layer Use the lasso tool to outline the.

Using Excel To help with data. Excel is a spreadsheet program that can interface with Word, or PowerPoint A spreadsheet program has cells (little blocks)

XP Tutorial 7 New Perspectives on Microsoft Windows XP 1 Microsoft Windows XP Working with Graphics Tutorial 7.

Click your mouse for next slide Flash – Introduction and Startup Many times on websites you will see animations of various sorts Many of these are created.

PowerPoint Basics (for Macs) 1. Before you start your project, you need: Completed project storyboard. Files with images, sound, or video already saved.

Learning PowerPoint Part Two: Working With Images Directions: Click the slide icon in the section at the right to move on to the next slide.

ACTIVINSPIRE TRAINING Tips and tools for creating Flipcharts on ActivInspire.

Introduction to Using the Notebook 10 Software for SMART Board Day 2 LIVINGSTON PARISH PUBLIC SCHOOLS Facilitated by S. Waltman.

CTS130 Spreadsheet Lesson 9 - Building Charts. What is a Chart? A chart is a visual display of information in a worksheet. Charts can help you make comparisons,

Show images on digital camera, select one and click open with… Photoshop. Camera Photos:

Project #1: Layers & Styles This lesson offers a step-by- step example that will help you master the art of layers. The power and flexibility of Photoshop.

Creating HUD Rings. Step 1 Open a new document in Illustrator. You can set it to the size you prefer to work in. In my case I have it set at 500px by.

BE1151 Image J Protocol For Western blot analysis 2/10/2006 Terry D. Johnson.

This is what we want to draw Extrude Mirror Round Edges.

1.To begin click: File, New, then select Design 2.Draw a circle, then use the dimension tool to set it’s size to 84 diameter or 42 radius. 3. Next draw.

Ext 10 Mr. Jean October 2 nd, The plan: Video clip of the day The Timeline.

To begin click: File, New, then select Design Familiarize yourself with the controls on the page. Then use the maximise button the make the window fill.

Design Studies 20 ‘Show Off’ Project How to make a computer monitor In Google Sketchup By: Liam Jack.

1 Using layers NOTES. 2 Open Adobe Photoshop Elements. Select a size of A4. Create 5 new layers by selecting layer > new. Do this action five times. Click.

Chris Jackson - Castle College - January 2008 Editing And Merging Two Images In Photoshop An Introduction To Basic Image Editing.

+ Christmas Village Ms. Hattenrath. + Christmas Coloring Sheets On the following slides, there will be Christmas coloring sheets. Please select the ones.

Objective: Students will be able to apply their knowledge of filters, and will learn about touch-up tools. Bellwork: Name a time, or application, where.

Foundation year Practical Lec. 4:Practical Lec. 4: Presentation Software Using Microsoft Office 2007 Practical Lec. 4:Practical Lec. 4: Presentation Software.

Year 9Dice. Select “New” Select “Metric” Standard (mm).ipt 1 2.

Fireworks Web Page mockups. Set up canvas/page size Fireworks is very well-suited to designing graphics and web pages for the screen. To create a web.

This is the finished body RADIUS LOFT EXTRUDE. Creating the body taper Open the wing Click on new part in context.

Chapter 2 Using Selection Tools and Shortcut Keys

Hyperstudio: A Beginner’s Tutorial By Judy Swaim.

Grade 4 Science.  -Click Start  -Click Programs  -Click IHMC Cmap Tools  -Click Cmap Tools.

Objective: Students will learn tools in Photoshop to manipulate images and make the unrealistic look possible. Bellwork: What are some unrealistic photos.

Adobe Photoshop CS5 Chapter 1 Editing a Photo. Start Photoshop and customize the Photoshop workspace Open a photo Identify parts of the Photoshop workspace.

This is what we want to create Add component and fix in place Add component and make it spin (Get propeller from the CD or use skills from previous tutorials.

Paint Tutorial Created February 2006 Start Paint: Start>Programs>Accessories>Paint.

The Lego Man Basic 3D Modelling. The Head Use the Revolve Tool to get the sketch from 2D to 3D.

Adobe Photoshop T.Ahlam Algharasi. Adobe Photoshop Adobe Photoshop is a seriously powerful photo and image edit ( treating and manipulation, compositing,

Interactive PPT Directions. IMPORTANT!  Before you make any hyperlinks or action buttons, you should create the slide that you want to link to and give.

Making Histograms with the TI-83 Plus Procedure Reference:

The Tail, this is what we want to make. Extrude Mirror Radius.

CIS 205—Web Design & Development Flash Chapter 3 Working with Symbols and Interactivity.

Adobe Photoshop CS5 Chapter 5 Enhancing and Repairing Photos.

Spreadsheet Enhancement Features Objective 6.02 Enhancement Features.

Working with Tables A table is a grid of cells arranged in rows and columns. Tables can be customized and are useful for various tasks such as presenting.

Quick Tips for Graphics Inserting Graphics From File 1. Click the Insert Menu. 2. Select Picture, and then click From File. 3. Navigate to the desired.

1.To begin click: File, New, then select Design 2.Next draw a rectangle. 3. Use the measuring tool to dimension the sides to 150mm by 75mm 4. Change to.

Adobe Photoshop CS5.

Spreadsheet Enhancement Features

+ – Fig. S1 – – – – + + Airianah et al. Orange G

Spreadsheet Enhancement Features

DIRECTIONS: 1. Click Enable Editing in the yellow bar above.

Program for interactive plotting of tri-linear data.

Chapter 1: Digital Communication Tools

TEXT FORMAT Textured and layered background with title - Advanced

Lab 08 Introduction to Spreadsheets MS Excel

Presentation transcript:

How to use Image J software in quantitative analysis of western blots

1. Open your file.

2. Under Image>Type click on 8-bit to convert the image to grayscale.

3. Go to the menu Process>Subtract Background. Try a rolling ball radius of 50. This removes some of the background coloration from your image.

4. Choose the Rectangular selection tool. Draw a rectangle around your first lane. Encompass some area of the lane above and below the band of interest. Press the 1 buttom (or go to Analyze>Gels>Select first lane). A new window will pop up with a copy of your image and a label over your first rectangular selection.

5. Use the arrow keys to move the rectangle over the next lane. Press 2 (or go to analyze> Gels>select next lane) to place a selection around the lane. Repeat this for each lane on the membrane, moving the box and pressing 2 to place the selection.

6. When finished, press 3 (or go to analyze>Gels>Plot lanes), which pops up a new window with a profile plot of each lane.

7. Choose the straight line selection tool. At the base of each peak, draw a line from one side of the peak to the other. This encloses the area of the peak. The tails to either side of the peak are the background signal.

8. When each peak has been closed off at the base with the straight line tool, choose the magic wand from the tool palette. Using the spacebar and mouse, drag the profile plot back down until you are at the top peak. With the wand, click inside the peak. Repeat this for each peak as you go down the profile plot. When each peak has been selected, go to Analyze>Gels>Label peaks. This labels each peak with its size expressed as a percentage of the total size of all the measured peaks. You can go to the results window and choose Edit> Copy All to copy the results for placing in a spreadsheet.