BE1151 Image J Protocol For Western blot analysis 2/10/2006 Terry D. Johnson.

Slides:

Advertisements

Similar presentations

September, 2004Patient Care Inquiry PCI - Customizing Your Patient ID Menu.

Advertisements

Outlining the Action By Brisa Waechter. Usually sport or action shots are best but you can use any photo you like.

GIMP Tutorial v2.0 Boo Virk

Drawing Objects with Illustrator 1.Start a new image in RGB mode. 2.Size 1024 X Unit = pixels 4.Go to View > Show Grid to turn on the grid. 5.Go.

XP New Perspectives on Microsoft Office Excel 2003, Second Edition- Tutorial 3 1 Microsoft Office Excel 2003 Tutorial 3 – Developing a Professional- Looking.

NI Multisim and Ultiboard

Using Draw Tools C 2012jkc. Click File on the menu bar and select Page Setup on the dropdown menu to get the Page Setup dialog box. Under the Margins.

Working with SharePoint Document Libraries. What are document libraries? Document libraries are collections of files that you can share with team members.

Created by Josh Ziegler

Google Earth How to create a Google Earth Tour and place it in your Wiki.

XP 1 Microsoft Office Excel Developing a Professional-Looking Worksheet.

InDesign CS3 Lessons 1 and 2. Work Area When First Opened.

Using Excel To help with data. Excel is a spreadsheet program that can interface with Word, or PowerPoint A spreadsheet program has cells (little blocks)

Making Tables and Graphs with Excel The Basics. Where do my IV and DV go? Just like you would create a data table on paper, your IV goes in the leftmost.

XP Tutorial 7 New Perspectives on Microsoft Windows XP 1 Microsoft Windows XP Working with Graphics Tutorial 7.

Forced Focus Tutorial. Here is a photo of the Adobe CS box in a tranquil background setting. The tranquil being the rocks, waterfall, flowers, morning.

OST Introduction to Excel Excel 2004 The Project Gallery is a window that links you to every application in Microsoft Office. It allows.

Microsoft Office Excel 2003 Tutorial 3 – Developing a Professional-Looking Worksheet.

Using the TI-83+ Creating Graphs with Data. Preparing to Graph  Once the calculator is on and you have entered data into your lists, press the “Y=“ button.

Starting screen for Google Sketchup Sketchup is used to make 3 dimensional objects 3 axis – Green is x axis Red is y axis Blue is z axis.

Welcome to Photoshop 5 Tutorials Welcome to Photoshop 5 Tutorials.

Calibration & Measurements. Calibrating the System Before we can make any measurements we need to calibrate our imaging system to create real world numbers.

PowerPoint Basics Tutorial 3: Graphics In this tutorial we’ll be looking at graphics, and the various types of illustrations that can be included in a.

Creating Buttons – Lesson 51 Creating Buttons Lesson 5.

Learning PowerPoint Part One: Working With Words Directions: Click the slide icon in the section at the right to move on to the next slide.

How to Create a PowerPoint Presentation. Starting PowerPoint Click 4 th grade folder Find power point in list Highlight and Click on.

How to use Image J software in quantitative analysis of western blots.

This is what we want to draw Extrude Mirror Round Edges.

Exploring the Macromedia Flash Workspace – Lesson 2 1 Exploring the Macromedia Flash Workspace Lesson 2.

PowerPoint Basics Tutorial 3: Graphics In this tutorial we’ll be looking at graphics, and the various types of illustrations that can be included in a.

To begin click: File, New, then select Design Familiarize yourself with the controls on the page. Then use the maximise button the make the window fill.

Using MS Photo Editor. Crop an image Click Select on the Standard toolbar, and then drag over the area of the image you want to keep. On the Image menu,

1 Using layers NOTES. 2 Open Adobe Photoshop Elements. Select a size of A4. Create 5 new layers by selecting layer > new. Do this action five times. Click.

GIS + ADOBE ILLUSTRATOR

Photoshop Editing Work Flow A Guide to the Full Meal Deal.

{ Aim: How can we process camera raw files?.  When you make adjustments to a camera raw image, Photoshop preserves the original camera raw file data.

Objective: Students will be able to apply their knowledge of filters, and will learn about touch-up tools. Bellwork: Name a time, or application, where.

Step 1 1.) go up to the Layer menu in the Menu Bar at the top of the screen, choose New, and then choose Layer via Copy.

Working with Shapes Guided Lesson. Objective  In this lesson you will learn how to insert a shape and format it by changing its fill color, outline color,

Excel Tips to Make Your Life Easier Michael Winecoff Associate University Librarian for Technical Services November 5, 2015.

1.Begin by opening VB 2.Click New Project or the icon Hello World Tutorial NOTE: depending on your version of VB – the images might be slightly different.

Objective: Students will learn tools in Photoshop to manipulate images and make the unrealistic look possible. Bellwork: What are some unrealistic photos.

This is how you invoke the Microsoft Visual Studio 2010 Software. All Programs >> Microsoft Visual Studio 2010.

Photoshop Actions Lights, Camera, Actions in Photoshop.

Adobe Photoshop T.Ahlam Algharasi. Adobe Photoshop Adobe Photoshop is a seriously powerful photo and image edit ( treating and manipulation, compositing,

Customizing Menus and Toolbars CHAPTER 12 Customizing Menus and Toolbars.

PhotoShop Creative Suite 3 Chapter 1 The Work Area.

CIS 205—Web Design & Development Flash Chapter 3 Working with Symbols and Interactivity.

Adobe Photoshop CS5 Chapter 3 Working with Layers.

1.To begin click: File, New, then select Design 2.Next draw a rectangle. 3. Use the measuring tool to dimension the sides to 150mm by 75mm 4. Change to.

Pagination Using MS Word for Windows to Insert Page Numbers.

Getting Started with Microsoft Word

V2.2 Boo Virk GIMP Tutorial v2.2 Boo Virk

Adobe Photoshop CS5.

+ – Fig. S1 – – – – + + Airianah et al. Orange G

Using Google “My Maps”.

Microsoft Word: Tables

Animated travel routes on a map (Intermediate)

Where to Access TE Items

Forced Focus Tutorial.

InDesign Basics.

Recording and editing a Podcast

Autodesk Inventor Tips and Tricks for New Users

Introduction To Photoshop

Navigating Excel.

How to use the snipping tool

Microsoft Outlook (Disabling desktop notifications)

Presentation transcript:

BE1151 Image J Protocol For Western blot analysis 2/10/2006 Terry D. Johnson

BE Open Image J and, in it, open the.TIF file to be analyzed - in this case, “example.tif”.

BE1153 1b. Optional If the image is not already an 8-bit.TIF, go to Image -> Type and select 8-bit. If the image is not dark enough to analyze properly, go to Image -> Adjust -> Brightness/Contrast and adjust the slider bars until the bands on the image are visible. The pixel values will not be changed, just the view.

BE In the main menu, select Analyze > Calibrate.

BE Select the Uncalibrated OD option from the Function: dropdown box, then click OK.

BE A logarithmic plot should appear; move it to the side, but do not close the window.

BE Go back to your.TIF image and select the Rectangle Tool from your toolbox. Box all of the bands of interest across all of the lanes and hit Crtl-H to bring up a histogram.

BE Carefully outline the first lane with the same tool. Go to Analyze > Gels > Select First Lane.

BE Drag the rectangle that you used for the first lane over to the next, and mark it with Analyze > Gels > Mark Next Lane. Don’t draw a new rectangle! You want every rectangle to have the same area. Repeat until all the lanes have been marked.

BE Click on Analyze > Gels > Plot Lanes. You’ll end up with an image file with plots for each of the lanes that you marked. The plot on top is the first lane that you marked. Use Image > Rotate 90 Degrees Right (or Left) and/or Image > Zoom > Out so you can see and work with all of the plots.

BE11511 (OPTIONAL) If you have more than one band per lane, you’ll end up with a plot for a single lane that has multiple peaks (one for each band). You’ll need to separate them into individual peaks with the Line Tool as demonstrated below on another image.

BE Select the Magic Wand tool and select each plot beginning with the first. A box will appear with the Area measurement for each plot as it is selected. The area of that histogram plot represents the darkness of each band in the gel.

BE Once you have selected each band, you can analyze the area measurements in your spreadsheet of choice.