Micropipettes Step-by-Step to use Micropipettes 1) Check the volume 2) Attach disposable tip (different tips for each size micropipette) 3) Depress the.

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Presentation transcript:

Micropipettes

Step-by-Step to use Micropipettes 1) Check the volume 2) Attach disposable tip (different tips for each size micropipette) 3) Depress the plunger (before you put in sample) to the first stop 4) Immerse tip in sample 5) Draw up the sample slowly and evenly, NOT all at once 6) Pause 7) Withdraw the tip 8) Dispense the sample along the inside wall of tube to help coax out the sample 9) Slowly depress plunger to second stop to expel last bit of fluid, and hold plunger down in the position 10) While holding plunger DOWN withdraw the pipette 11) Release plunger 12) Discard the tip *Always Change tips for each new reagent you need to pipette or if you touch any other liquids. We want to remember our ASEPTIC techniques!

300µl (p1000 pipette)

120µl (p200 pipette)

50µl (p200 pipette)