Anesthetic Modulation of Protein Dynamics: Insight from an NMR Study Presented by: Logan Woodall Mentor: Dr. Pei Tang.

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Presentation transcript:

Anesthetic Modulation of Protein Dynamics: Insight from an NMR Study Presented by: Logan Woodall Mentor: Dr. Pei Tang

Mistic A 4  helix bundle in the transmembrane domain of Cys-loop family of receptors

Experimental Setup 1 H- 15 N TROSY-HSQC spectra used to determine dissociation constants STD tests to confirm direct Mistic- anesthetic interaction Magnetic pulse sequences used to study protein dynamics

Sites of Halothane Interaction Remarkable chemical shifts after exposure to halothane Certain residues’ signals were also either strengthened (i.e. S58) or weakened (i.e. I52)

TROSY-HSQC Overlay

Sites of Interaction (Contd) Most helical regions unaffected while loop regions generally interacted with halothane Binding likely disrupts interaction between different loops or residues within the same loop

Sites of Interaction (Contd) Regions of significant chemical shift localized to residues near/in loops

Halothane Effects on Structure Relatively small rmsd values indicate conservation of structure upon halothane addition

Halothane Effects on Motion R 2 relaxation rates affected in loops connecting helices and along  2 helix (T39- N45) Residues T39, N45, and Y82 underwent chemical exchange on mico/millisec time scale (- halothane) but did not undergo chemical exchange (+ halothane)

Effects on Motion (Contd) Two pairs of nearby residues w/ similar exchange rates affected similarly by halothane addition Most relaxation dispersion values decreased by halothane Some residues' chemical shifts not affected by halothane while dynamics were (I52, Y82)

Key Points of Study Residues with motion on microsecond/millisecond timescale mostly in loops between helices Residues near each other spatially (but not necessarily sequentially) display concerted motion

Key Points (Contd) Halothane seemingly capable of altering Mistic slow dynamics through both direct interaction with residues and allosteric modulation General anesthetics tend to interact preferentially with amphipathic regions of proteins