Lasers and Optical Characterization Lab Ed Van Keuren, Changbao Ma, Kristen Perlot, David Littlejohn, John Menzel, Johnny Rogers, Mike Lavoie, Jonathan.

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Lasers and Optical Characterization Lab Ed Van Keuren, Changbao Ma, Kristen Perlot, David Littlejohn, John Menzel, Johnny Rogers, Mike Lavoie, Jonathan Flyer GAEL: Mak Paranjape, John Currie, Ali Leeds, Drew Monica SAIC: Tom Schneider, Robb White BASF: Wolfgang Schrof Dept. of Chemistry: Jen Swift ChemImage: Pat Treado, Matt Nelson

Nanoparticle synthesis and characterization Imaging using molecular probes MEMS/MOEMS

Nanoparticle synthesis and characterization Aggregation/crystallization of organic nanoparticles in binary solvents Fluorescence correlation spectroscopy Nanocomposite OLEDs

Nanoparticle nucleation Solvent shifting: Nucleation induced by exchange of solvent with miscible non-solvent (micronization, reprecipitation, drowning out crystallization) Rapid (injection) or slow (ternary phase transition)

Nanoparticle nucleation I: Clear solution II: Bulk precipitate Solution (water- miscible solvent) Water

Nanoparticle nucleation How do single molecules come together in solution to form nanoparticles? Transition from distribution of unstable clusters to nucleation sites Cluster size distribution Cluster lifetimes

Spectrally selective FCS Fluorescence Correlation Spectroscopy (FCS) Minute sampling volume defined by confocal optics Low concentration – to M Temporal variation in fluorescence emission due to number fluctuations, changes in chemical species Autocorrelation function of fluorescence can yield diffusion coefficients, reaction rates

Spectrally selective FCS Perylene: long wavelength bands in nanocrystals diffusion of the aggregates only. MgPC: fluorescence quenching ( nm) in nanoparticles will allow diffusion coefficients of single molecular species to be isolated.

Polymer nanoparticles Monomer nanoparticles formed by rapid injection into nonsolvent, which induces aggregation, followed by initiation of free radical polymerization.

Imaging using molecular probes Three dimensional thermal imaging 2 photon FRAP Pattern photobleaching

Thermal imaging in tissue 3-d fluorescence imaging + temperature dependent dye  measurement of temperature profiles near MEMS heaters

Pattern photobleaching Pattern created using two photon photobleaching Can follow micro- deformations in polymer films, for example, water absorption

MEMS/MOEMS Detection of biomarkers by competitive binding in microchannels 2-d protein separations chip Thermo-optic waveguide switches

Optical biomarker detection with competitive binding Development of a MEMS device containing both optical waveguides and microfluidic channels Single mask Competitive binding of analyte with tagged receptors for fluorescence detection

Optical biomarker detection with competitive binding