International PSC Study Group Meeting, Oslo, Jun 2010 Cholangiocarcinoma in PSC: Opportunities within a study group Dr Steve Pereira Institute of Hepatology UCL Medical School & University College Hospital

Characterisation of serum proteins in biliary tract cancer, PSC and IAC using 2D-DIGE and LC-MS/MS Neomal S Sandanayake 1,2,3, John Sinclair 3, Fausto Andreola 2, Michael H Chapman 1,2, Stephane Camuzeaux 3, George J Webster 1,2, Ian D Norton 4, Ross C Smith 5, John F Timms 3, Stephen P Pereira 1,2 1 Department of Gastroenterology and Hepatology, University College London Hospital; 2 Institute of Hepatology, University College London; 3 Institute for Women's Health, University College London; 4 Department of Gastroenterology, 5 Department of Surgery, Royal North Shore Hospital, University of Sydney, Australia Conclusions: Differentially expressed serum proteins were identified in BTC compared to PSC, IAC and healthy controls using serum immuno- affinity depletion, 2DDIGE and LC-MS/MS.

Proposal Suspicious biliary strictures Imaging Bile / brushings Blood Tissue Clinical follow for > 1 yr in patients without proven malignant diagnosis Malignancy Benign disease Biobank

Patients: >18 yrs Benign or suspected or proven malignant PB disease Written informed consent Samples: Biliary brushings and bile - in storage buffer (provided) Blood - NIH SOPs - inverted 5 times, 1 hour clot at room temp, centrifuged 2200rpm at 4°C, serum aliquoted into 1.5ml cryovials, -80° C Tissue samples - snap-frozen in liquid N 2 and stored at -80°C - FFPE TRANSBIL: TRANSlational research in BILiary tract cancer + Proposal

 Pre-clinical samples  202,000 women - annual phlebotomy  270 individuals developed pancreatic cancer  54 individuals developed biliary tract cancer UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) serum bank at UCL Resource

Cholangiocarcinoma in PSC Opportunities for trials? Surgery / OLT Stents Radiotherapy / Brachytherapy Chemoradiation Chemotherapy PDT

April