Biotic controls on microbial communities in estuarine and riverine habitats Trisha Pascal-Lopez Microbial Ecology Lab, Mentors: Rima Franklin, Ph.D, and.

Slides:

Advertisements

Similar presentations

Exploring transient phytoplankton spring blooms across lakes to understand the significance and control of temporal patterns in freshwater bacteria Bertilsson,

Advertisements

AP Environmental Science Chapters 17 and 18. * Eutrophic Lake.

Lab: Plankton Sampling and Analysis Ventura Pier, Harbor and Estuary.

Genetic Profiling of Oncoid Stromatolites from the Phosphorus- Limited Rio Mesquites in Cuatro Cienegas, MX Alisa Glukhova NASA Space Grant Symposium April.

Chapter 3.  The study of interactions between organisms and their environment.

Preliminary Results No nutrients 5 psu 2 psu 0.5 psu + Nutrients 5 psu 2 psu 0.5 psu Foodweb support for the threatened Delta smelt: Salinity effects on.

Bacteria-Phytoplankton Competition Overview : Bacterial immobilization or remineralization of N. Competition between bacteria and phytoplankton for DIN.

The Dead Zone: The Dead Zone: Hypoxia in the Gulf of Mexico.

Foodweb support for the threatened Delta smelt: Microzooplankton dynamics in the low salinity zone of San Francisco Bay J.K. York 1, B. Costas 1, G. McManus.

What is limiting marine heterotrophic microorganisms ? Johnny Berglund Marine ecology Department of Ecology and Environmental Science Umeå University.

Streams (Rivers) Stream = water flowing downhill in a defined channel. Amount of flow significant. Usually varies seasonally. (wet season & dry season)

Approach: Networked Aquatic Microbial Observing System Approach: Networked Aquatic Microbial Observing System Results: Data From Both Networked Sensors.

Foodweb support for the threatened Delta smelt: Phytoplankton production within the low salinity zone Ulrika Lidström 1, Anne Slaughter 1, Risa Cohen 2,

Microbial Processes and Material Flow in Ocean Ecosystems.

2.D.1 Biotic and Abiotic Interactions

Chapter 3 Review.

DR. PAUL A. BUKAVECKAS VIRGINIA COMMONWEALTH UNIVERSITY Developing water quality standards to Protect the James River against Impacts from Algal Blooms.

OptionEcology and Conservation: G1 Community Ecology.

Dissolved Oxygen and Primary Productivity Lab

Ecosystems and Communities Chapter 20. The role of Climate  What is climate? –Temperature, precipitation, other environmental factors combine to produce.

ECOLOGY. What is Ecology? What do living things have in common? Life characteristics:  made up of cells  reproduction  based on a universal genetic.

The Brunner Island Power Plant: The Seasonal Effects of a Generated Thermal Plume on the Phytoplankton, Periphyton, and Macroinvertebrate Populations of.

What is Ecology?.

POPULATION ECOLOGY. ECOLOGY Study of living organisms as groups Interactions between living organisms (predator-prey, parasitism etc) Interactions between.

Chapter 3: The Biosphere

ECOLOGY. ECOLOGY The study of the ABUNDANCE, DISTRIBUTION, and DIVERSITY of organisms and their interactions within an environment.

Unit 2 Ecology Ch. 3 The Biosphere. What Is Ecology?  Like all organisms, we interact with our environ.  To understand these interactions better & to.

Bellwork: 09/05/2012 1)Change 10 to 25% of the water within your tank. 2)If you are not helping your group change water, you need to be finishing the fish.

PH DO DIP TDP Bethany Remeniuk, Department of Biology, York College of Pennsylvania Is Microcystis aeruginosa an Initiator in a Positive Feedback Cycle.

Chapter 3: The Biosphere

Water Quality in Local Lakes and Ponds Aquatic Ecology Unit.

Mussel mediation of nutrient availability and algal composition 1.How do dreissenid mussels affect phosphorus (P) availability? 2. How do they affect seston.

Bacterial production and Microsystin in Lake Taihu GUANG GAO Nanjing Institute of Geography & Limnology, CAS

Physical Properties Discharge rate, temperature and other physical attributes of the stream.

©MathScience Innovation Center Our Backyard Waterways : Eutrophication Presented by: Rachel Martin Day 2.

Nutrient Dynamics Nutrient Uptake and Growth Models

Population Dynamics Introduction

Diversity and Functional Variation of Denitrifying Bacterial Communities in the Cape Fear River Estuary Brian Shirey Marine Biology.

TMDLs and Statistical Models Kenneth H. Reckhow Duke University.

ABSTRACT Species in natural communities are linked together by the transfer of energy and nutrients. We investigated the effects of top predators on nutrient.

January 27, 2011 Summary Background on Delta Flow and Habitat Relationships Delta Stewardship Council Presentation by the Independent Consultant.

Great Lakes Environmental Research Laboratory Review – Ann Arbor, MI November 15-19, Click to edit Master text styles –Second level Third level.

Ecology Accelerated Biology. Ecology o o Ecology – The scientific study of interactions among organisms and between organisms and their physical environment.

ECOLOGY 1. WHAT IS ECOLOGY OBJECTIVES: 3.1 Identify the levels of organization that ecologists study. Describe the methods used to study ecology. 2.

Ecology Study of the relationships between organisms and their environments Study of the relationships between organisms and their environments Their interactions.

The Nature of Ecology. How to make a food web. 1.Start with one producer on BOTTOM and draw arrows up to the things that eat them (their predators). (**

The Impact of Nutrients on Picophytoplankton Populations Along the Atlantic Coast Melinda Norris and Dr. Jessica Nolan Conclusions  The phytoplankton.

 Salinity: Salinity is the concentration of dissolved salts in the water and is an important element of a  habitat. Aquatic animals are adapted to living.

2. Name two of the four biogeochemical cycles.

Surface Water Quality Indicators Around the Farm Water Quality Area of Expertise Team.

Organisms and Their Relationships

Eutrophication Eutrophication.  What is Eutrophication?  Step by step Eutrophication process  Effects on water quality, treatment costs, compliance.

1 Trophic levels in Ecosystems Food Chains, Food Webs, Energy Pyramids, Biological magnification.

Unit Living Things and the Environment Section 21.1 Organisms obtain food, water, shelter, and other things it needs to live, grow, and reproduce.

Ecology. Scientific study of the interactions between __________ and their environment.

ECOSYSTEM COMPONENTS: ENERGY FLOW & MATTER CYCLING.

Vocabulary:  Eutrophication: is a natural process; where lakes, as they age, build up nutrients which cause phytoplankton to grow.  Phytoplankton: Microscopic.

Ecology 1.Ecosystems 2.Biomes 3.Ecosystem Interactions 4.Cycles of Matter 5.Ecosystem Response to Change Resources 1.Skim Chapters Vocab Chart.

EUTROPHICATION By: Annette Miles.

Fundamentals of Ecology

Watersheds Review Science 8 SOL.

Science Word Wednesday, 9/6/17

Types of Water Pollution

By Pete Kahn Mentors: Lydie Herfort and Peter Zuber

Ch 52: Intro to Ecology and the Biosphere

AQUATIC ECOSYSTEMS Part One: Freshwater.

AIR BIOLOGY REVIEW Ecology.

Mrs. Brostrom Oceanography

Presentation transcript:

Biotic controls on microbial communities in estuarine and riverine habitats Trisha Pascal-Lopez Microbial Ecology Lab, Mentors: Rima Franklin, Ph.D, and Amy Jenkins

Nutrient Input (ex. farm runoff) Algal Bloom (consumed by bacteria) Bacteria (hypoxia) Marine creature die Trophic cascade

A. Purpose –Study the ecological relationships that occur in an aquatic microbial food web –To understand the interdependence of three levels of the food web (phytoplankton, bacteria, and grazers) –Focus on response of bacteria to other community members

1) Two different initial environment samples: James River mile 21 (Estuarine) James River Rice Center (Riverine) B. Setup Temp (C) Salinity (ppt) Turbidity (NTU) pHDO (mg/L) Estuarine site (RM 21) Rice Center (RM 75) Courtesy of Paul Bukaveckas & River Ecology lab

i)EnvironmentPhytoplanktonBacteriaGrazers ii)Environment + cycloheximide PhytoplanktonBacteria iii)Environment + dark BacteriaGrazers iv)Environment + cylcoheximide + dark Bacteria ) Four treatments: Manipulated light and dark levels Eukaryotic growth inhibitor (cycloheximide) 3 replicate of each treatment per environment, 3 day incubation B. Setup

1) Phytoplankton community: Fluorescence detection of chlorophyll levels 2) Bacterial community: Abundance (microscopic direct counts) Culturability (counts of solid media/agar) Genetic profiling (RAPD DNA fingerprints) 3) Grazer community: Abundance and diversity (microscopic determination) Mainly protozoa, rotifers and larvae Methods

C. Results Initial comparison Distinct communities at start of experiment Estuary had lower phytoplankton, grazer and bacteria abundance than riverine site (Rice). Estuarysite RiceCenter

Were the treatments effective? Changes to the phytoplankton population (greater for estuary) Affected estuary & riverine phytoplankton differently Reduced grazer abundance Grazers linked to phytoplankton? Decrease in grazer diversity

How did bacteria respond to the changes in the phytoplankton and grazer abundance/ diversity? Estuary: Bacteria abundance not linked to phytoplankton or grazers abundances (unaffected in all treatments). Rice Center (Riverine): Treatments had an effect on bacterial community.

Changes in bacterial community composition? Estuary: Community composition similar in all treatments Light Dark + cyclohex. Dark Light + cyclohex. River: Changes in bacterial community with each treatment. Light Dark + cyclohex. Dark Light + cyclohex.

Implications Microbial communities in the Estuarine and Riverine environments are different. –Estuarine environment bacterial communities are not linked to grazer or phytoplankton abundance –Riverine environment shows bacterial interactions with phytoplankton and grazer communities Microbial community interactions vary depending on the environment which controls their response to environmental stress.

D. Future Goals –Statistical analysis of data –Chemistry (Nitrogen and DOC) –Run the experiment again with changes Add another mile marker Collect water sample at different seasons or times of day Manipulate nutrient concentration

Thank You Questions?