Loschmidt Laboratories Enantis, Ltd. PROTEIN ENGINEERING

LOSCHMIDT LABORATORIES & ENANTIS  17 years experience in protein science  multi-disciplinary team

LOSCHMIDT LABORATORIES & ENANTIS  17 years experience in protein science  multi-disciplinary team  laboratories and facilities (1.5 mil EUR) University Campus and Biotechnology Incubator, Brno

LOSCHMIDT LABORATORIES & ENANTIS  17 years experience in protein science  multi-disciplinary team  laboratories and facilities (1.5 mil EUR) University Campus and Biotechnology Incubator, Brno  molecular biology and microbiology

LOSCHMIDT LABORATORIES & ENANTIS  17 years experience in protein science  multi-disciplinary team  laboratories and facilities (1.5 mil EUR) University Campus and Biotechnology Incubator, Brno  molecular biology and microbiology  bioinformatics and molecular modelling

 17 years experience in protein science  multi-disciplinary team  laboratories and facilities (1.5 mil EUR) University Campus and Biotechnology Incubator, Brno  molecular biology and microbiology  bioinformatics and molecular modelling  biochemistry and biophysics LOSCHMIDT LABORATORIES & ENANTIS

BIOSENSING DECONTAMINATION BIOCATALYSIS

DEVELOPMENT OF BIOCATALYTIC PROCESS

 GENETIC DATABASES  EXTREMOPHILES DEVELOPMENT OF BIOCATALYTIC PROCESS

 DIRECTED EVOLUTION resistance to organic solvents DEVELOPMENT OF BIOCATALYTIC PROCESS

 DIRECTED EVOLUTION  RATIONAL DESIGN thermostability enhancement DEVELOPMENT OF BIOCATALYTIC PROCESS

 DIRECTED EVOLUTION  RATIONAL DESIGN thermostability enhancement DEVELOPMENT OF BIOCATALYTIC PROCESS

 DIRECTED EVOLUTION  RATIONAL DESIGN thermostability enhancement 20°C DEVELOPMENT OF BIOCATALYTIC PROCESS

 OPTIMIZED EXPRESSION  FERMENTATION  DOWN STREAM DEVELOPMENT OF BIOCATALYTIC PROCESS

 MEDIUM ENGINEERING  IMMOBILISATION DEVELOPMENT OF BIOCATALYTIC PROCESS

HOTSPOT WIZARD Hot Spot Wizard RCSB PDB user

Hot Spot Wizard RCSB PDB CSA UniProt user PDBSWS HOTSPOT WIZARD

Hot Spot Wizard RCSB PDB CSA UniProt user CAVER CASTp PDBSWS HOTSPOT WIZARD

Hot Spot Wizard RCSB PDB CSA UniProt user CAVER CASTp PDBSWS tunnels Cd-hit BLASTNRDB MUSCLE Rate4Site HOTSPOT WIZARD

low high mutability HOTSPOT WIZARD

1,3-1,4-  -glucanase PDB-ID 1GBG haloalkane dehalogenase PDB-ID 1BN6 phosphotriesterase PDB-ID 1I0D  -lactamase PDB-ID 1BLS HOTSPOT WIZARD

Estimated mutability hot spots region Proportion of mutations HOTSPOT WIZARD positive and neutral

Estimated mutability hot spots region Proportion of mutations HOTSPOT WIZARD positive and neutral slightly negative

positive and neutral slightly negative deleterious Estimated mutability hot spots region Proportion of mutations HOTSPOT WIZARD

Pavelka et al. Nucl. Acids Res. 37: W