Adventures in HRP Ellyn Daugherty San Mateo Biotech Career Pathwaywww.SMBiotech.com EMC/Paradigm Publishingwww.emcp.com/biotech VWR/Sargent-Welchwww.sargentwelch.com

San Mateo Biotechnology Career Pathway Giving motivated teenagers and adults the skills to pursue several career and academic options.

Partnerships with Industry and Academia

Biotechnology is an opportunity to teach science in a way that results in science literacy & research skills & career awareness for virtually every student. Students learn the skills and gain the confidence and self-directedness that are necessary for independent research(science fair projects) and higher-level science courses. Students see meaning and application in their math, biology and chemistry. Students learn the science and business of biotech so that they are better prepared to make decisions about their futures and society.

Text - Chapter 6 and 14 Lab Manual - Labs 6f-6h, 14a Student CD Tutorials for basic skills Course Planner/Instructor’s Guide The HRP Activities are found in Chapter 6

Biotech 3 HRP labs: Peroxidase Assay HRP extraction from HR Root HRP assay using PAGE HRP assay w/ TMB indicator HRP used in ELISAs

How Do You Make the Phosphate Buffer? You need 100 mL of a 25 mM NaH 2 PO 4 * H 2 0, pH 7 buffer g of NaH 2 PO 4 * H 2 0 is measured measured on a balance and put into an appropriate vessel. Then, solvent is added up to the 100 mL graduation g NaH 2 PO 4 * H mL add solvent (dH 2 0) to 75 mL Adjust pH to 7 and add dH 2 0 to 100 mL conc. desired X volume desired X molecular weight = amount of solute in mol/L in L in g/mol in g mol/L X 0.1 L X 138 g/mol = g NaH 2 PO 4 * H 2 0 Convert 25 mM = mM and convert 100 mL = 0.1 L

50 kD 35 kD HRP is 43 kD

My Professional Goals Biotechnology programs in every high school, CTE center, and community college, tailored to the student population and industry and community needs Aligned and articulated programs in HS and CC so that teenagers and adults have the same platform from which to enter specialized programs To make it as easy as possible for educators to do the other 2

Get help starting or improving a biotech program ! Ellyn Daugherty SM Biotech Career Pathway JEFF GANDY Holly Ahern Sandy Duffey for preview copy