The University of Jordan Faculty of Medicine Gram-negative coccobacilli and cocci Prof. Dr. Asem Shehabi and Dr. Suzan Matar.

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The University of Jordan Faculty of Medicine Gram-negative coccobacilli and cocci Prof. Dr. Asem Shehabi and Dr. Suzan Matar

1-Haemophilus Group  Gram-negative cocco/large- thin bacilli.. Micro- aerophilic.. Requires growth factors ( V- X- factors)..Red blood cells, Grow around hemolytic Staphylococcus, Rapid autolysis outside body  Normal Flora.. Human Upper Respt. Tract.. Many species Haemophilus.. opportunistic pathogens Virulence factors.. Endotoxin & Capsule.. localized- invasive infections  H. influnzae type b: Most common pathogenic species.. capsulated.. Causing Localized and Invasive Infect.. Sore Throat, Otitis Media, Sinusitis, Conjunctivitis, Brochopneumonia, Septicemia & Meningitis.. Children 6 Months-5 Years.. Antibiotics. Lab Diagnosis: Blood, CSF & Others Culture.. Chocolate & blood agar included X & V Factors.. Hib- Vaccine.. Infants > 2 months old. Lab Diagnosis: Blood, CSF & Others Culture.. Chocolate & blood agar included X & V Factors.. Hib- Vaccine.. Infants > 2 months old.

Haemophilus colonies growth surrounding Stapholococcus colonies

Bordetella pertussis  Gram-negative coccbacilli.. Aerobic.. Highly human Communicable agent.. Droplets Infection..close contact.. Bacteria attach to lower respiratory tract mucosa., Incub. period 7-10 days..Destruction tracheal ciliated cells by releasing Pertussis toxin (cytotoxin). Pertussis start by mild coughing, sneezing..intense cough (Whooping / paroxysmal cough), vomiting, red eyes..Infants & Children more susceptible to infection than adults, high fatal in Adults than in children. Antibiotics are useful in first stage. Prevention: DTP vaccine within first Ms  Diagnosis: Clinical signs and Symptoms.. Less Culture & Lab tests.

Neisseria & Moraxella Groups  Neisseria spp., Moraxella spp.: Gram-negative diplococci, Facultative anaerobes, Oxidase & Catalase+ve, Highly susceptible to Low/High Tempt… Dryness, Rapid Autolysis.. Room Tempt. Normal Flora Respiratory Tract.. Rare Non-pathogens (N. sicca, N. flava, M. Mucosa).. Common Pathogens.. N. gonorrhea, N. meningitidites.  1-N.gonorrhea: Pili, IgA-Protease, LPS, Colonization Mucosa.. Invasion..Inflammation.. Genitourinary Tract, Rectum, Throat, Sexually Transmitted Diseases.. Acute/Subacute/Asymptomatic Infections.. Uretral/Vaginal Discharge, Urethritis, Cervicitis, Salpengitis.. Common Reinfection..

Neisseria Gramstain-Intracellular Presence in Urethral Discharge

Follow/2  Lab diagnosis: Direct Gram-stain.. Intracellular G-ve diplococci in WBCs (pus cells).. Rapid Culture in Blood/chocolate Agar.. Antibiotics, No Vaccine. 2- N. meningitidites: Capsular Polysaccharides, LPS, IgA-Protease, Serotypes A,B,C, Invasive, Exogenous Infect. Respt.Tract.. Sore Throat.. Septicemia.. Meningitis.. Acute disease with high Mortality without treatment. Children (6-Months-5 years) more susceptible than adults. Epidemic outbreaks.  Protective Vaccine is available.  3- M. catarrhalis: Part of normal Respiratory tract.. Opportunistic pathogen.. Pneumonia.. Rarely Septicemia.. Compromised Lung/heavy smokers.  Lab Diagnosis: Direct-Gram-stain, Culture Blood/ chocolate Agar, Biochemical tests, Antibiotics,