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Lecture 6 Web: pollev.com/ucibio Text: To: Type in:

Folding proteins Need precise shape – active site Amino acid sequence – determines folding Secondary structural elements form

Motifs & Domains Motif: ______________________ Eg. Helix-turn-helix Will not fold independently

Motifs & Domains Domain:________________ Eg. Ig domain Folds independently

Working together Quaternary structure Multiple peptide chains interact Structure & function dependent on interactions

Quaternary structure

Another protein folding experiment… APX Normal activity250 units450 units +Heat5 units9 units +Heat +recovery188 units14 units +Protein “Y”244 units412 units +“Y” + heat7 units11 units +heat +Y +recovery198 units388 units

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Sequential stabilization of intermediates

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The importance of protein structure “Chaperones” & protein folding Misfolded proteins are very, very bad! E.g. Huntington’s disease

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The importance of structure “Chaperones” & protein folding Misfolded proteins are very, very bad! E.g. Huntington’s disease E.g. Prions