Control of protein affinity in bioactive nanocellulose by passivation with PDMAEMA- block-POEGMA copolymers and false positive response Maija Vuoriluoto.

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Presentation transcript:

Control of protein affinity in bioactive nanocellulose by passivation with PDMAEMA- block-POEGMA copolymers and false positive response Maija Vuoriluoto 1, Hannes Orelma 1, Leena-Sisko Johansson 1, Baolei Zhu 2, Mikko Poutanen 3, Andreas Walther 2, Janne Laine 1, Orlando J. Rojas 1 1 Bio-based Colloids and Materials (BiCMat), Department of Forest Products Technology, Aalto University, School of Chemical Technology, Espoo, Finland 2 DWI − Leibniz-Institute for Interactive Materials Research, Aachen, Germany 3 Department of Applied Physics, School of Science, Aalto University, Espoo, Finland

Motivation Non-specific protein adsorption limits the accuracy and selectivity of biosensors Multifunctional polymers containing poly(ethylene glycol) (PEG) molecules effective in preventing non-specific surface protein adsorption Cellulosic materials are appealing substrates for biomedical applications

Structure of PDMAEMA-block- POEGMA copolymers analyzed RAFT polymerization of: PDMAEMA = poly(2-(dimethylamino)ethyl methacrylate) Quaternized by methyl iodide POEGMA = poly(oligo(ethylene glycol)methyl ether methacrylate) Highly hydrophilic due to PEG units 3 D 33 -EGMA 52 D 33 -EGMA 137 D 58 -EGMA 118 D 58 -EGMA 10 D 74 -EGMA 17 D 74 -EGMA 118 PDMAEMA POEGMA

Reduction in human IgG adsorption on TEMPO-CNF with blocking agents 4 TEMPO-CNF (REF) D 33 -EGMA 52 D 33 -EGMA 137 D 58 -EGMA 10 D 58 -EGMA 118 D 74 -EGMA 17 D 74 -EGMA 118 SuperBlock BSA PEG-amine Blocking efficiency % 96 % 100 % 96 % 88 % 85 % 96 % 15 % 23 % 75 % Adsorbed amount after blocking with

Thank you!