Viral Inhibition of the Anaphase- Promoting Complex/Cyclosome as a Mechanism of Tumor-specific Cell Death Jose G. Teodoro, Ph.D McGill Cancer Center Department.

Slides:

Advertisements

Similar presentations

10.3 Workbook Corrections.

Advertisements

p53 Revealed character as a tumor suppressor gene in 1989.

The Story of Bcl-2 Linking cell apoptosis to tumor metastasis Crystal structure of Bcl-2 complex By Yaming Wang.

MDM2: Oncogene Chan Lee. Discovery of MDM2: starting with tumor suppressor p53.

Retinoic Acid Receptor Alpha and Acute Promyelocytic Leukemia Nidhi Thapar April 1, 2004.

C22 Cancer and the Immune System Cell births ~ cell deaths; production of new cells is regulated Cells that do not respond to normal growth controls can.

APOPTOSIS “Cell Death” By Brian Abadie, Emily Anderson, andJohn Ramsey.

What is Cancer? How it occurs and cell cycle regulation.

New insights into the mechanisms of action of microtubule targeting agents that are effective against metastatic breast cancer Susan L. Mooberry, Ph.D.

10.3 Regulating the Cell Cycle

Cancer and Apoptosis combined An historical introduction Mathieu Noteborn LIC, Leiden University, NL LC Workshop - 29 October 2007.

Regulating the Cell Cycle

Infectivity Enhanced Adenovirus as a Strategy for Improving the Efficiency of RNA Interference in an Ovarian Cancer Model T Michael Numnum, MD International.

Circulating Cell-Free DNA Necrosis, apoptosis, and possibly “active secretion” are thought to be the mechanisms by which cell-free nucleic acids enter.

KEY CONCEPT Cell cycle regulation is necessary for healthy growth.

Section 10.3 (Pg ): Regulating the Cell Cycle

Lesson Overview Lesson Overview Regulating the Cell Cycle Lesson Overview 10.3 Regulating the Cell Cycle.

10.3 Regulating the Cell Cycle

Therapeutic miRNA Delivery Suppresses Tumorigenesis in a Murine Liver Cancer Model Janaiah Kota,1 Raghu R. Chivukula,2 Kathryn A. O’Donnell,3,4 Erik A.

Inhibition of PDCD6 Induces Cell Proliferation and Reduces Apoptosis in Human Epithelial Ovarian Cancer Cells Yan Huang, Xiaohua Wu Department of Gynecology,

Prostate Cancer – a model of Epigenetic Catastrophe? A Perry et al,, End. Rel. Cancer 2006.

Cancer Accelerated Biology. Learning Objectives The different methods of diagnosing cancer. The difference between a malignant tumor and a benign tumor.

Cell Cycle Checkpoint.

10.3 Regulating the Cell Cycle

Cancer Cancer- a malignant tumor; the result of abnormal cell proliferation. Regulation of Cell Division –Tumor Supressor Genes Genes that inhibit cell.

Cancer What is it? How does it happen? Any way to treat it?

10.3-Regulating the Cell Cycle Controls on Cell Division The cell cycle is controlled by regulatory proteins both inside and outside the cell. Cyclins.

Regulating The Cell Cycle. Warm Up – The Cell Cycle The cell spends 80% of the time in _______________ and 20% of the time in ________________ What are.

Cancer Cells. Cell Review What kind of cell is this bacterial cell? What are the types of cells plants have?

Modulation of NF ‐ κB ‐ dependent transcription and cell survival by the SIRT1 deacetylase by Fan Yeung, Jamie E Hoberg, Catherine S Ramsey, Michael D.

10.3 Regulating the Cell Cycle

Mouse Double Minute 2 (MDM2)

Figure 1. Resistance mechanism against first generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI). (A) Mutations in the EGFR.

10.3 Regulating the Cell Cycle

Prolyl‐hydroxylase‐domain proteins regulate hypoxia inducible factor‐α in response to O2 availability. Prolyl‐hydroxylase‐domain proteins regulate hypoxia.

Targeting the PARP DNA repair pathway enhanced cytotoxicity induced by chemotherapy. Targeting the PARP DNA repair pathway enhanced cytotoxicity induced.

IDH1 mutant glioma cells are sensitive to TMZ

10.3 Regulating the Cell Cycle

10.3 Regulating the Cell Cycle

Figure 1 Cellular processes involved in cancer development

An endothelial metronome breast cancer?

NF-κB: A Coordinator for Epigenetic Regulation by MLL

Losing Control of the Cell cycle

Multidimensional Drug Profiling By Automated Microscopy

Regulating the Cell Cycle

Whipping NF-κB to Submission via GADD45 and MKK7

Mechanisms of apoptosis and growth arrest by isothiocyanates.

Volume 104, Issue 6, Pages (March 2001)

Bcl-2: A Matter of Life-or-Death

Notes: Regulating the Cell Cycle

10.3 Regulating the Cell Cycle

10.3 Regulating the Cell Cycle

Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,

Schematic model of effector pathways that mediate tumor suppression by p53. Schematic model of effector pathways that mediate tumor suppression by p53.

High correlation of expression changes of NMD-regulated genes identified by both the pSILAC screen and previously reported global RNA screens after UPF1.

Altered pathways in prostate cancer.

Putative targets of miRNAs directly induced by p53 with down-regulated mRNA- and de novo protein synthesis or reduced de novo protein synthesis only. Putative.

Bar plot representation of the transcriptomic changes in Δsaci_ptp and Δsaci_pp2a. Bar plot representation of the transcriptomic changes in Δsaci_ptp and.

Apoptosis Cell Volume 108, Issue 2, Pages (January 2002)

Antibody specificity ascertained by 2D-PAGE Western immunoblotting (IEF) of total cellular protein extracts from the RT4 human bladder cancer cell line.

10.3 Regulating the Cell Cycle

10.3 Regulating the Cell Cycle

Peptide mass spectra of SUMO target proteins.

Hypothetical model of HvAP2 control of stem elongation.

Flavopiridol inhibits rhabdoid cell survival and induces cell cycle arrest and apoptosis. Flavopiridol inhibits rhabdoid cell survival and induces cell.

Schematic representation of signaling pathways associated with cannabinoid receptor activation induced by its agonists. Schematic representation of signaling.

Mechanism by which flavopiridol induces cell cycle arrest and apoptosis in rhabdoid tumor cells. Mechanism by which flavopiridol induces cell cycle arrest.

Cyclooxygenase (COX) independent chemopreventive mechanisms of non-steroidal anti-inflammatory drugs (NSAIDs). *Genes with a T cell factor 4 responsive.

Senescence Elicits Stemness: A Surprising Mechanism for Cancer Relapse

Presentation transcript:

Viral Inhibition of the Anaphase- Promoting Complex/Cyclosome as a Mechanism of Tumor-specific Cell Death Jose G. Teodoro, Ph.D McGill Cancer Center Department of Biochemistry McGill University Montr é al, Canada

Viruses as tools in the study of cell transformation and apoptosis

Apoptin localizes in a transformation-dependent manner

Apoptin localization is regulated by a factor in transformed cells

Proteomic analysis of Apoptin-associated proteins

Subunit structure of the APC/C Thornton et al. Genes & Dev (2006)

Apoptin induces destabilization of the APC/C FPLC analysis

Apoptin colocalizes with APC/C within PML bodies

Tran et al. J Virol Oct 17

Overview and future directions How does G2/M arrest proceed to apoptosis? Do current chemotherapies rely on APC/C inhibition for efficacy? Can the APC/C be an anti-cancer drug target?

Acknowledgements McGill University Isabelle Gamache Mohamed Mustafa Russell Towers Sarah Assadian Yiqing Lu Michael Hell U Mass Michael Green Destin Heilman Albert Parker Julie Zhou Reagents Frank Graham Crister Hoog