Parts of a Light Microscope

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Presentation transcript:

Parts of a Light Microscope Source: http://hrsbstaff.ednet.ns.ca/holmesdl/Biology2011/PartsofaLightMicroscopeandcalculations.ppt

How to Focus on Specimen Turn on light source on high Turn the objective to low (4x) Lower the objective lens so that it is close but not touching the slide Center the image by moving the stage Adjust the diaphragm for optimum light Adjust focus, first with course then fine Switch to higher power, adjust ONLY with fine focus

How to Use a Microscope http://www.youtube.com/watch?v=bGBgABLEV4g

Field of View The diameter of the circle of light you can see when looking through the microscope.

Usually measured in mm but expressed in μm Field of View Con’t Measured by placing a clear ruler under the objective and counting how many mm fit across the diameter Usually measured in mm but expressed in μm

Field of View Con’t Typical field of view measurements for light microscope: Objective Lens Total Mag. Field of View (mm) Field of view (μm) 4x 4.5 10x 1.8 40x 0.45

Estimating Size under Microscope Estimating the size of a specimen using field of view diameter… Estimated Length (actual length) = Field diameter (μm) Fit number Fit number is the amount of times the specimen can fit across the diameter

Estimating Size Con’t What would be the fit number here?

Calculating Diagram Magnification When drawing specimens viewed under the microscope it is custom to calculate the magnification of your drawing To do this, you must first calculate estimated length of specimen under microscope. Diagram (drawing) Magnification = Diagram size (μm) Estimated Length of Specimen NOTE: Measure your drawing in mm then convert to μm

Calculating Diagram Magnification Use this diagram to answer questions 1, 2, 3 on sheet Total Mag = 400x