IUFRO Division 5 Conference 5.07.B Energy from biomass / Chemicals from wood Chi-Lin Wu and Shang-Tzen Chang National Taiwan University

Isolation and Identification of Antifungal Constituents from the Heartwood Extracts of Taiwan Hinoki (Chamaecyparis obtusa var. formosana) C.-L. Wu & S.-T. Chang IUFRO Division 5 Conference 5.07.B Energy from biomass / Chemicals from wood School of Forestry and Resource Conservation, National Taiwan University National Taiwan University

Aim of work and methods Taiwan hinoki (Chamaecyparis obtusa var. formosana) is an endemic species in Taiwan and its timbers are well recognized for their natural decay resistance. Isolation and identification of antifungal constituents from the heartwood extracts of Taiwan hinoki were carried out in this study. Bioassay-guide fractionation Agar dilution assay Column chromatography HPLC MS, 1 H-NMR, 13 C-NMR, and 2D-NMR analyses

(100 μg/mL) Results Ethanolic Extract Taiwan Hinoki Heartwood n-Hex fr. EtOAc fr. EtOH fr. CFH 1CFH 6CFH 12 Extracted by EtOH Liquid-liquid partition Column chromatography : Silica gel, n-Hex / EtOAc / MeOH HPLC separation : Si-60 column, n-Hex/EtOAc ～～ (500 μg/mL) T-Muurolol α-Cadinol Control α-Cadinol

Heartwood extract exhibited an excellent antifungal activity against brown-rot fungus L. sulphureus and white-rot fungus L. betulina. According to MS, 1 H-NMR and 13 C-NMR analyses, 16 compounds were identified, namely α-cadinol,  -cadinol, T-muurolol,  -costol, hinokiol, hinokione, sandaracopimaric acid… etc. α-Cadinol and T-muurolol efficiently inhibited the growth of wood-rot fungi. Conclusions National Taiwan University