Synthesis of Perbenzoylated Carbohydrates in Preparation for Anthocyanin Pigment Characterization Brandon Lew March 15, 2006.

Slides:

Advertisements

Similar presentations

Synthesis and Analysis of Aspirin Chemistry 1060 Laboratory.

Advertisements

Porphyrin Research at the Evergreen State College: The Synthesis, Separation, Isolation and Characterization of Di-functional meso-substituted Porphyrins.

The most important instrumental technique used by organic chemists to determine the structure of organic compounds. NMR spectroscopy helps to identify.

The Constructions of Stable Nucleic Nanostructures Anna Feng, Tamika Roberts Dr. James Canary Johan Guillaume NYU Department of Chemistry.

Characterization of Anthocyanins in Purple Pods of Phaseolus vulgaris cv. Blauhilde Brandon Lew March 24, 2007.

Analysing the METABOLOME 1.Metabolite Extraction 2.Metabolite Separation 3.Metabolite detection (with or without separation) 4.Data analysis.

Protein structure determination. Tertiary protein structure: protein folding Three main approaches: [1] experimental determination (X-ray crystallography,

CHEM 515 Spectroscopy Lecture # 1.

The Identification and Characterization of the Cardioactive Principals in Hawthorn Extract by Niel Henriksen Mentor: Dr. Phil Proteau College of Pharmacy.

Synthesis of Oil of Wintergreen

Introduction to Natural Products Chemistry Cells of organisms - plants, fungi, bacteria, lichens, insects, animals - produce a large variety of organic.

Organic Chemical Analysis (of organic compounds).

Drs. Wei Tian & Yanhui Chen Sep-Dec Main Content Introduction of Nuclear Magnetic Resonance (NMR) Analysis One Dimensional NMRs 1 H NMR 13 C NMR.

PHARMACEUTICAL CHEMISTRY RESEARCH PROJECTS 2013 ;.

Year 12 Chemistry Unit 3 – AOS 1 Chemical Analysis.

Effects of 2,4-D on Anthocyanin Pigments in Red Norland Potatoes (Solanum tuberosum) Shannon Engelman 2007.

Forensic Application of Fluorescence

COLUMN CHROMATOGRAPHY (CC). TLC - Optimizing for column chromatography Optimum: 0.2 < R f < 0.5.

COLUMN CHROMATOGRAPHY

Title: Lactose Lab. Introduction: Background :Enzymes are important components of digestion because they break down large food molecules into smaller.

Suzuki Coupling: Aqueous and Anhydrous Synthesis of Ferrocene-Capped Thiophene Bill Mitchell

Carbohydrate & Lipid Review. 1. Can you name this structure?

Synthesis of Biologically Active Thiadiazole Analogs Lillian Nordahl 2006.

MODULE 2 PART II: BIOCHEMISTRY OF MILK: Introduction to Lipids, Proteins (discussed in Module 1), and Carbohydrates. OBJECTIVES: 1.KNOW CHARACTERISTICS.

Investigation of Anomalous Effects in the Deprotonation and Alkylation of Ketones Patrick Kelly-Goss 2004.

State that the mobile phase may be a liquid or a gas.

Chemistry 2412 L Dr. Sheppard

The 2p-3d Electron Transition Multiplet of Ar +13 : A Stellar Density Diagnostic Laura Heeter Kristina Naranjo-Rivera

NMR and The Designing and Manufacturing of an NMR Probe for 109 Ag in Li x Ag 3 V 4 O 11 Cathode Material, for 0.72

Synthesis of New Scaffolds via Bisalkyne Cyclizations Catalyzed by Triflic Acid Jaime Alvarez Duque, Kyle Strom, John K. Snyder Boston University, Department.

Disaccharides Di- is a Greek prefix meaning two. Disaccharides Carbohydrate made up of two monosaccharides linked together Carbohydrates are inorganic.

Pasta vs Candy???. ORGANIC COMPOUND Carbohydrates.

The most important instrumental technique used by organic chemists to determine the structure of organic compounds. NMR spectroscopy helps to identify.

Construction of Stable Nucleic Acid Nanostructures Anna Feng Dr. James Canary Johan Guillanme NYU Department of Chemistry.

Why do people eat a bowl of pasta before a race??? !

Carbohydrates are the main source of energy, comes from plants and some in milk. 3 types are sugars, starches and fiber. Plants make all of these. Photosynthesis.

Nitrification Inhibition (NI) compounds from Brachiaria humidicola, a tropical grass Gopalakrishnan, S.*, Subbarao, G.V., Nakahara, K. and Ito, O. Japan.

Introduction to Natural Products Chemistry Cells of organisms - plants, fungi, bacteria, lichens, insects, animals - produce a large variety of organic.

Refining the Structure of cADPR Rodney Agnant and Dr. Steven M. Graham Harlem Children Society and St. John’s University.

MOLECULAR STRUCTURE ANALYSIS NMR Spectroscopy VCE Chemistry Unit 3: Chemical Pathways Area of Study 2 – Organic Chemistry.

Instrumental Analysis

DRUG CHECKING TUTORIAL

Analytical Chemistry II ChEm 321

(Instrument part) Thanundon Kongnok M

S. B. Osman, O. Korostynska, A. Mason, J. D. Cullen and A. I

Carbohydrates Disaccharides Polysaccharides.

Electromagnetic Radiation

Nuclear magnetic resonance NMR spectroscopy is a key analytical technique for structure elucidation of a wide range of materials from small molecules to.

Introduction of Combinatorial Chemistry

Nuclear Magnetic Resonance NMR Spectroscopy Nuclear Magnetic Resonance NMR Spectroscopy Shovan Sarker Biochemistry & Moleculer Biology SUST.

Chapter 31 & 32: Separation Science & Chromatography

HIGH PRESSURE LIQUED CHROMATOGRAPHY (HPLC)

Carbohydrates Disaccharides Polysaccharides.

חיפוש שיטות מחקריות להוכחת נוכחות התרופה בדם כאשר

Biomass composition and lignin chemistry of energy sorghum

Theory of NMR In a magnetic field, there are now two energy states for a proton: a lower energy state with the nucleus aligned in the same direction as.

Nuclear Magnetic Resonance Spectroscopy

Why do people eat a bowl of pasta before a race???

Mass Spectrometry.

Standard 6 Food Science Carbohydrates.

December 15, 2015 Mitchell Hamline School of Law | 2.

What is chromatography?

Pharmaceutical Analytical Techniques

Advanced Pharmaceutical Analysis

Darrell Clinton and H. Dorota Inerowicz Purdue University

Instrumentation.

The kynurenic pathway. The kynurenic pathway. The encircled metabolites were measured and symbols indicate method and biological sample, that is, LC-MS/MS.

Sera metabolite profiles of patients with RA discriminate rituximab responders and non-responders. Sera metabolite profiles of patients with RA discriminate.

Mass spectrometry (MS) is an analytical technique that can be used to determine the mass, elemental composition or chemical structure of molecules. Mass.

Presentation transcript:

Synthesis of Perbenzoylated Carbohydrates in Preparation for Anthocyanin Pigment Characterization Brandon Lew March 15, 2006

Introduction Purpose: To develop a technique for carbohydrate characterization through NMR so that anthocyanin carbohydrate tails may be characterized.

Anthocyanins

Carbohydrate Tails Anthocyanidin Base

Anthocyanin Characterization NMR of anthocyanins Seeram et al Separation of tails Takeoka et al Combine to perform NMR on separated tails

Carbohydrates ChairChain

Perbenzoylation Levene and Meyer (1927) Glucose D’Accorso et al. (1981) NMR data of many monosaccharides

Perbenzoylation (Protocol 1) β-glucose Perbenzoylated-β-glucose

Purification Thin-layer chromatography (TLC) Column chromatography High-pressure liquid chromatography (HPLC)

Nuclear Magnetic Resonance Spectroscopy (NMR) 1 H/ 13 C EM radiation Excited State Ground State Radio wave Detector Magnetic Field

Protocol 1 Results: 1 H NMR of perbenzoylated β glucose

Protocol 1 Results: HPLC of perbenzoylated maltose

Protocol 1 Results: 13 C NMR of perbenzoylated maltose

Perbenzoylation (Protocol 2) β-glucose Perbenzoylated-β-glucose

Protocol 2 Results: 1 H NMR of perbenzoylated β glucose

Protocol 2 Results: HPLC of perbenzoylated β glucose

Procedure 2 Results: 1 H NMR of perbenzoylated α glucose

Conclusion Protocol 1 – contaminated Some disaccharides selectively benzoylated β-glucose Perbenzoylated-β-glucose

Conclusion Protocol 2 – purer, quicker Perbenzoylation works for characterization β-glucose Perbenzoylated-β-glucose

Future Work Use perbenzoylation to characterize carbohydrate tails of anthocyanins Anthocyanins differ by their tails Important for developing anti-oxidant food additives

Acknowledgements Dr. Matachek Hamline University Ms. Fruen Ms. Richter, Bill Mitchell Team Research

Synthesis of Perbenzoylated Carbohydrates in Preparation for Anthocyanin Pigment Characterization Brandon Lew March 15, 2006