Ancient Lake non-isolated pothole River where pupfish can swim from pothole to pothole. isolated potholes Hypothesis is that there was an ancient lake.

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Ancient Lake non-isolated pothole River where pupfish can swim from pothole to pothole. isolated potholes Hypothesis is that there was an ancient lake filling a basin. Current Lake The lake dried up leaving potholes where pupfish live now. Isolated potholes do not connect and non isolated potholes are connected by rivers.

PCR Polymerase Chain Reaction

Allows us to amplify a particular segment of DNA- over and over. Forensics- amplify tiny amount until large enough to use.

Ingrediants Template DNA Taq Polymerase Primers dNTPs Buffer Magnesium Ions