Recombinant DNA technology 13.2

Genetic Engineering  Genetic engineering is a fast more reliable method to increase the frequency of a gene in a population

 Recombinant DNA is created by connecting or recombining pieces of DNA from different sources

Transgenic organisms  Contain DNA from another (foreign) source

Creating recombinant DNA  Restriction enzymes cut both strands of a DNA molecule at a specific nucleotide sequence  This creates pieces or fragments of DNA that contain specific genes

 A vector (virus, plasmid, micropipette, etc) transfer the DNA fragment to the new organism, or host

 Vectors do not always take the DNA fragments easily, so another enzyme is used to attach the fragments to the vectors

Gene cloning  A clone is a genetically identical copy  Gene clones are used for recombinant DNA and other processes such as DNA fingerprinting

Polymerase Chain Reaction  This is the process of using an enzyme to make copies of fragments of DNA

Applications of DNA technology  What are some applications for recombinant DNA in each of the following areas? Industry Medicine Transgenic animals Agriculture