The Arabinose Operon Gene Regulation. Why Gene Regulation? Developmental Changes Cell Specialization Adaptation to the environment Prevents creation of.

Slides:

Advertisements

Similar presentations

In May, students in the Biology Department produced WHS’ first Genetically Modified Organism... More detailed science Experimental results Overview Photo.

Advertisements

Module based on a kit from Bio-Rad Laboratories, Inc. Thank you to :

Preparing an Overnight Culture of Escherichia coli

Structure & Function of DNA. DNA and RNA are nucleic acids that consist of long chains of nucleotides The nucleotides have three parts; 1.Phosphate 2.Nitrogen.

Exercise 17 Bio 112 Genetics Lab. There are four grain phenotypes in the above ear of corn: Purple & Starchy(A), Purple & Sweet(B), Yellow & Starchy(C)

Operon and Plasmid Review. The control of gene expression Each cell in the human contains all the genetic material for the growth and development of a.

Genetic Analysis of Lac Operon Make partial diploids to do complementation tests: 1 copy of lac operon on E. coli chromosome. 2nd copy of lac operon on.

PGLO™ Transformation. Central Framework of Molecular Biology DNA RNA ProteinTrait.

pGLO Transformation LAB AP LAB 6 BIO-RAD lab book pGLO ori bla GFP araC.

90- How can we make more insulin? V How can we make more insulin? By Transforming Bacteria V

Genetic engineering Genetic engineering: manipulating DNA or organisms to perform practical tasks or provide useful products We’re going to look at the.

GFP Transformation Lab Images taken without permission from

Biotechnology Explorer Program Serious About Science Education.

pGLOTM Bacterial Transformation Courtesy BioRad Corporation

PGLO™ Transformation and Purification of Green Fluorescent Protein (GFP)

pGLO™ Transformation and Purification of

Mrs. Stewart Medical Interventions Central Magnet School

Bacterial Transformation Lab “pGLO”

Today: Regulating gene expression in bactria Exam #1 T 2/17 in class Available: F and M 10-11am, noon-2pm, after 3pm T after 2pm.

By Hewan Wami Bridges 2014 Prof. Sara F.Feinman Prof. Heather Kundzicz June 13, 2014.

Gene Expression and Regulation

This Little Light of Mine: This Little Light of Mine: Transform bacteria with a Jellyfish gene to make them glow Module based on a kit from Bio-Rad Laboratories,

PGLO ™ & GFP. Central Framework of Molecular Biology DNA RNA ProteinTrait.

Overview Amgen Biotech Labs In this set of labs, students will:

Transformation of E. coli with Green Fluorescent Protein (GFP)

Hey Kim, this site shows the kids everything! NY ‘09 use this for the prelab stuff!! Michael.Gregory/files/Bio%20100/Bio%

Transforming E. coli with a Recombinant Plasmid. What is biotechnology? Employs use of living organisms in technology and medicine Modifying living organisms.

Bioengineering Turning Genes on and off in Bacteria.

Restriction Analysis of pDRK and pGRN Original Plasmids

Bacterial Genetics & Transformation

Bacterial Plasmids Loops of DNA found in some bacteria

In the pGLO lab, we will: Use recombinant DNA Genetically engineer E. coli bacteria by inserting a plasmid Plate and grow bacteria Determine if the proteins.

Moving Green Fluorescent Protein

Liquid cultures to Lysis: Movie Clip from Teachers domain Student Guide Lab 6.

pGLO and Ampicillin Resistance Transformation of E. coli

GFP Transformation Lab

Genetic Transformation of Bacteria and Gene Regulation.

Transport Nucleus Cytoplasm Protein gene DNA mRNA The Cell:

pGLO™ Transformation and Purification of Green Fluorescent Protein (GFP)

CAMPBELL BIOLOGY Reece Urry Cain Wasserman Minorsky Jackson © 2014 Pearson Education, Inc. TENTH EDITION CAMPBELL BIOLOGY Reece Urry Cain Wasserman Minorsky.

In the pGLO lab, we will: Use recombinant DNA Genetically engineer E. coli bacteria by inserting a plasmid Plate and grow bacteria Determine if the proteins.

PGLO Bacterial Genetics. E. Coli structure.

PGLO Transformation LAB AP LAB 6 BIO-RAD lab book pGLO ori bla GFP araC.

pGLO™ Transformation and Purification of

Bacterial Transformation. Chromosome? A long piece of DNA with many pieces of information on it, each piece is a set of directions for making a protein.

GFP Transformation Lab

pGLO™ Transformation and Purification of

Genetics of Bacteria Bacterial genome =.

Accelerated Biology Transformation Lab

pGLO Transformation LAB AP BIO LAB 6

Genetic Transformation of Bacteria and Gene Regulation

pGLO Transformation LAB AP Investigation 8

Gene Expression 1. Gene expression is the activation of a gene that results in transcription and the production of mRNA. Only a fraction of any cell’s.

Biorad pGlo-Biotechnology

Chapter 12.5 Gene Regulation.

PGLO Lab Purpose: To transform E. coli bacteria by adding plasmids that allow the bacteria to glow green under UV light in the presence of arabinose sugar.

PGLO Transformation.

Accelerated Biology Transformation Lab

Gene Expression Cage Match!!!

Transport Nucleus Cytoplasm Protein gene DNA mRNA The Cell:

Transformation Procedure Overview

Bacterial Transformation

pARA-R Sequence RFP Expression Sequence Biotechnology Lab Program

Prokaryotic (Bacterial) Gene Regulation

Biology II Study Guide for Unit Test on Operons and Transformation Lab 2013 You should be able to … 1. describe gene expression by explaining the following:

Abridged Genetic Engineering Pathway (Original “A” Sequence)

Gene Regulation pGLO Transformation.

Unit 5 Day 3 Lab Results.

GFP Transformation Lab

Presentation transcript:

The Arabinose Operon Gene Regulation

Why Gene Regulation? Developmental Changes Cell Specialization Adaptation to the environment Prevents creation of unneeded product

Arabinose Sugar Can be used for food E. coli make three enzymes needed to break down arabinose These enzymes are only made (genes are only expressed) when the arabinose is present.

Regulation of the Arabinose Operon The Operon AraBAra AAra D AraC pBAD promoter initiates transcription for all three genes Ara C is a DNA Binding Protein Arabinose AraC RNA Pol P BAD

PGLO Plasmid The Operon GFP AraC pBAD promoter initiates transcription Ara C is a DNA Binding Protein GFP is Green Fluorescent Protein Arabinose AraC RNA Pol P BAD

References Biotechnology Explorer, pGLO Bacterial Transformation Kit. BioRad.