Desired Gene Restriction Enzymes Bacterial defense against viral DNA Bacterial defense against viral DNA Cut DNA at specific sequences Cut DNA at specific.

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Desired Gene Restriction Enzymes Bacterial defense against viral DNA Bacterial defense against viral DNA Cut DNA at specific sequences Cut DNA at specific sequences CCTTTG AATTCCCAGAATC GGAAACTTAA GGGTCTTAG AATTCGGCCATATACG GCCGGTATATGCTTAA Restriction Sites for EcoRI

Electrophoresis Separation of molecules based on size Separation of molecules based on size Negatively charged DNA molecules are pulled through a gel by an electrical field Negatively charged DNA molecules are pulled through a gel by an electrical field Smaller molecules travel faster and farther Smaller molecules travel faster and farther

DNA Fingerprinting Variations in DNA Variations in restriction sites Variations in fragment sizes Variations in electrophoresis bands

Walter Jordan Poop

Gene Cloning Binary Fission Gene Splicing Transformation

Mammalian Cloning Somatic cell nuclear transfer

Stem Cells

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